Development of a PEI-coated SWNTs Nanocarrier for efficient delivery of CRISPR/Cas9 in early embryos of<i> Litopenaeus</i><i> vannamei</i>

doi:10.1016/j.aquaculture.2023.740424

IOCAS-IR

	Development of a PEI-coated SWNTs Nanocarrier for efficient delivery of CRISPR/Cas9 in early embryos of Litopenaeus vannamei
	Men, Jia L.1; Zhang, Yu T.1; Pei, Yue B.1; Li, Na 1; Xiang, Jian H.2; Zhou, Hai L.1,3,4
	2024-02-25
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	581 页码:14
通讯作者	Zhou, Hai L.([email protected])
摘要	Cultivation of superior varieties is the key to maintaining the sustainable development of the Litopenaeus van-namei (L.vannamei) industry. CRISPR/Cas9 technology represents a generation of genetic breeding technology based on gene editing. However, the conventional delivery strategy of CRISPR/Cas9 components could not be used due to the particular physiological traits and practical difficulties of L. vannamei embryos. We designed and established polyethylenimine (PEI)-coated nanoparticles with carboxylated SNWTs core to safely deliver CRISPR/Cas9 plasmids into early embryos for target gene editing. The results showed that the transfection ef-ficiency of this strategy was 36%, which was approximately 4-fold higher than the efficiency of the classical lipid transfection method. The transcription factor Pax6, which has notable effects on early embryonic eye develop-ment, provides clear phenotypic proof for this strategy. Unnatural base alterations were found in up to 20% of transfected embryos. This study establishes a foundation for the application of CRISPR technology in L. vannamei and provides an innovative approach for large-scale gene function studies in aquaculture.
关键词	Litopenaeus vannamei Nanocarrier CRISPR/Cas9 gene editing Transfection Early embryos
DOI	10.1016/j.aquaculture.2023.740424
收录类别	SCI
语种	英语
资助项目	Hainan Province Science and Technology Special Fund[ZDYF2022SHFZ321]; Graduate Innovation Research Project of Hainan Province[Qhyb2022-44]; Hainan Basic and Applied Basic Research Program (Natural Sciences) (High-level Talents Project)[2019RC077]; National Natural Science Foundation of China[42377273]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:001139843700001
出版者	ELSEVIER
WOS关键词	HATCHING ENVELOPE FORMATION ; DETERMINATION GENE NETWORK ; BLACK TIGER SHRIMP ; CARBON NANOTUBES ; PENAEUS-MONODON ; IN-VITRO ; DNA ; BACTERIA ; PAX6 ; POLYETHYLENIMINE
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184291
专题	中国科学院海洋研究所
通讯作者	Zhou, Hai L.
作者单位	1.Hainan Univ, Sch Life Sci, Haikou 570228, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Hainan Univ, One Hlth Inst, Haikou 570228, Hainan, Peoples R China 4.Hainan Univ, State Key Lab South China Sea Marine Resource Util, Haikou 570228, Peoples R China
推荐引用方式 GB/T 7714	Men, Jia L.,Zhang, Yu T.,Pei, Yue B.,et al. Development of a PEI-coated SWNTs Nanocarrier for efficient delivery of CRISPR/Cas9 in early embryos of Litopenaeus vannamei[J]. AQUACULTURE,2024,581:14.
APA	Men, Jia L.,Zhang, Yu T.,Pei, Yue B.,Li, Na,Xiang, Jian H.,&Zhou, Hai L..(2024).Development of a PEI-coated SWNTs Nanocarrier for efficient delivery of CRISPR/Cas9 in early embryos of Litopenaeus vannamei.AQUACULTURE,581,14.
MLA	Men, Jia L.,et al."Development of a PEI-coated SWNTs Nanocarrier for efficient delivery of CRISPR/Cas9 in early embryos of Litopenaeus vannamei".AQUACULTURE 581(2024):14.