Comparative transcriptome analysis reveals growth and molecular pathway of body color regulation in turbot (Scophthalmus maximus) exposed to different light spectrum

doi:10.1016/j.cbd.2023.101165

IOCAS-IR > 实验海洋生物学重点实验室

	Comparative transcriptome analysis reveals growth and molecular pathway of body color regulation in turbot (Scophthalmus maximus) exposed to different light spectrum
	Wu, Lele1 ; Sun, Wen 1; Zhou, Jiale 1; Li, Yaolin 1; Li, Jun 2; Song, Zongcheng 3; Song, Changbin 4; Xu, Shihong 2; Yue, Xinlu 3; Li, Xian 1,5
	2024-03-01
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
ISSN	1744-117X
卷号	49 页码:12
通讯作者	Li, Xian([email protected])
摘要	Fish body color changes play vital roles in adapting to ecological light environment and influencing market value. However, the initial mechanisms governing the changes remain unknown. Here, we scrutinized the impact of light spectrum on turbot (Scophthalmus maximus) body coloration, exposing them to red, blue, and full light spectra from embryo to 90 days post hatch. Transcriptome and quantitative real-time PCR (qRT-PCR) analyses were employed to elucidate underlying biological processes. The results showed that red light induced dimorphism in turbot juvenile skin pigmentation: some exhibited black coloration (Red_Black_Surface, R_B_S), while others displayed lighter skin (Red_White_Bottom, R_W_B), with red light leading to reduced skin lightness (L*) and body weight, particularly in R_B_S group. Transcriptomic and qRT-PCR analyses showcased upregulated gene expressions related to melanin synthesis in R_B_S individuals, notably tyrosinase (tyr), tyrosinase-related protein 1 (tyrp1), and dopachrome tautomerase (dct), alongside solute carrier family 24 member 5 (slc24a5) and oculocutaneous albinism type II (oca2) as pivotal regulators. Nervous system emerged as a critical mediator in spectral environment-driven color regulation. N-methyl D-aspartate (NMDA) glutamate receptor, and calcium signaling pathway emerged as pivotal links intertwining spectral conditions, neural signal transduction, and color regulation. The individual differences in NMDA glutamate receptor expression and subsequent neural excitability seemed responsible for dichromatic body coloration in red light-expose juveniles. This study provides new insights into the comprehending of fish adaptation to environment and methods for fish body color regulation and could potentially help enhance the economic benefit of fish farming industry.
关键词	Body color Light spectrum Melanin Regulation Scophthalmus maximus
DOI	10.1016/j.cbd.2023.101165
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41976122]; National Natural Science Foundation of China[32273104]; Young Talent Program of Ocean University of China[862201013156]; Agriculture Research System of China[CARS-47]
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:001127179400001
出版者	ELSEVIER SCIENCE INC
WOS关键词	SKIN COLOR ; NEURAL CREST ; RED PORGY ; R PACKAGE ; PIGMENTATION ; EXPRESSION ; EVOLUTION ; BEHAVIOR ; NUCLEUS ; PAGRUS
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184168
专题	实验海洋生物学重点实验室
通讯作者	Li, Xian
作者单位	1.Ocean Univ China, Fisheries Coll, Key Lab Mariculture, Minist Educ, Qingdao 266001, Peoples R China 2.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 3.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China 4.Chinese Acad Sci, Inst Semicond, Beijing 100083, Peoples R China 5.Ocean Univ China, Fisheries Coll, Qingdao 266001, Peoples R China
推荐引用方式 GB/T 7714	Wu, Lele,Sun, Wen,Zhou, Jiale,et al. Comparative transcriptome analysis reveals growth and molecular pathway of body color regulation in turbot (Scophthalmus maximus) exposed to different light spectrum[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2024,49:12.
APA	Wu, Lele.,Sun, Wen.,Zhou, Jiale.,Li, Yaolin.,Li, Jun.,...&Li, Xian.(2024).Comparative transcriptome analysis reveals growth and molecular pathway of body color regulation in turbot (Scophthalmus maximus) exposed to different light spectrum.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,49,12.
MLA	Wu, Lele,et al."Comparative transcriptome analysis reveals growth and molecular pathway of body color regulation in turbot (Scophthalmus maximus) exposed to different light spectrum".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 49(2024):12.