Stable carbon isotopic composition of amino sugars in heterotrophic bacteria and phytoplankton: Implications for assessment of marine organic matter degradation

doi:10.1002/lno.12468

	Stable carbon isotopic composition of amino sugars in heterotrophic bacteria and phytoplankton: Implications for assessment of marine organic matter degradation
	Guo, Jinqiang 1,2,3; Achterberg, Eric P.2; Shen, Yuan 4,5; Yuan, Huamao1,3,6,7 ; Song, Jinming1,3,6,7 ; Liu, Jin 8; Li, Xuegang 1,3,6,7; Duan, Liqin 1,3,6,7
	2023-11-22
发表期刊	LIMNOLOGY AND OCEANOGRAPHY
ISSN	0024-3590
页码	12
通讯作者	Yuan, Huamao([email protected]) ; Song, Jinming([email protected])
摘要	Compound-specific isotope analysis has opened up a new realm for resolving the sources and transformation processes of marine organic matter. However, the stable carbon isotope patterns of amino sugars remain unknown. We examined delta 13C of amino sugars in marine phytoplankton and heterotrophic bacteria, and the variations in amino sugar delta 13C during 66-d planktonic organic matter degradation experiments, to investigate the metabolic sources and transformations of amino sugars by bacterial reworking. The delta 13C values of glucosamine (GlcN) and galactosamine (GalN) were comparable in heterotrophic bacteria (difference Delta delta 13CGlcN-GalN = 0.4-4.0 parts per thousand) but pronouncedly different in phytoplankton (Delta delta 13CGlcN-GalN = 4.3-16.6 parts per thousand), suggesting similar synthesis pathways of GlcN and GalN in bacteria that differed from phytoplankton. Compared to GlcN and GalN, bacteria preferentially use isotopically light organic compounds for muramic acid (MurA) synthesis. During simulated microbial degradation of organic matter, the delta 13C difference between GlcN and GalN decreased from 5.8 parts per thousand on the initial day to 1 parts per thousand at a late stage in the experiment, but the difference between GlcN and MurA remained at 5.3 parts per thousand. This difference is consistent with the pattern in cultured phytoplankton (average Delta delta 13CGlcN-GalN = 5.9 parts per thousand +/- 1.4 parts per thousand) and heterotrophic bacteria (average Delta delta 13CGlcN-MurA = 4.6 parts per thousand +/- 3.4 parts per thousand), indicating enhanced bacterial resynthesis as degradation proceeded. Based on the difference in delta 13C among GlcN, GalN, and MurA, we propose a novel index of variation in amino sugar delta 13C, representing amino sugar resynthesis, to describe the diagenetic state of organic matter. Together, these findings suggest that amino sugar delta 13C can be used as a new tool to track heterotrophic processes of marine organic matter.
DOI	10.1002/lno.12468
收录类别	SCI
语种	英语
资助项目	Fujian Provincial Central Guided Local Science and Technology Development Special Project; China Scholarship Council (CSC); Deutscher Akademischer Austauschdienst (DAAD); National Natural Science Foundation of China[42276038]; National Natural Science Foundation of China[42322602]; National Natural Science Foundation of China[42106040]; National Natural Science Foundation of China[LSKJ202204001]; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050501]; Shandong Provincial Natural Science Foundation[ZR2020YQ28]; Two-Hundred Talents Plan of Yantai Scholar Project Special Funding; Fundamental Research Funds for the Central Universities of China[20720210076]; [2022L3078]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:001107700000001
出版者	WILEY
WOS关键词	NITROGEN ISOTOPES ; MURAMIC ACID ; FRACTIONATION ; PARTICULATE ; DELTA-C-13 ; OCEAN ; BIOSYNTHESIS ; PACIFIC ; SEA ; MONOSACCHARIDES
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183976
专题	海洋生态与环境科学重点实验室
通讯作者	Yuan, Huamao; Song, Jinming
作者单位	1.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao, Peoples R China 2.GEOMAR Helmholtz Ctr Ocean Res Kiel, Marine Biogeochem Div, Kiel, Germany 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen, Peoples R China 5.Xiamen Univ, Coll Ocean & Earth Sci, Xiamen, Peoples R China 6.Laoshan Lab, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 7.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 8.Chinese Acad Sci, Inst Oceanol, Publ Technol Serv Ctr, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Guo, Jinqiang,Achterberg, Eric P.,Shen, Yuan,et al. Stable carbon isotopic composition of amino sugars in heterotrophic bacteria and phytoplankton: Implications for assessment of marine organic matter degradation[J]. LIMNOLOGY AND OCEANOGRAPHY,2023:12.
APA	Guo, Jinqiang.,Achterberg, Eric P..,Shen, Yuan.,Yuan, Huamao.,Song, Jinming.,...&Duan, Liqin.(2023).Stable carbon isotopic composition of amino sugars in heterotrophic bacteria and phytoplankton: Implications for assessment of marine organic matter degradation.LIMNOLOGY AND OCEANOGRAPHY,12.
MLA	Guo, Jinqiang,et al."Stable carbon isotopic composition of amino sugars in heterotrophic bacteria and phytoplankton: Implications for assessment of marine organic matter degradation".LIMNOLOGY AND OCEANOGRAPHY (2023):12.

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