ROS-induced moderate autophagy of haemocytes confers resistance of<i> Mercenaria</i><i> mercenaria</i> to air exposure stress

doi:10.1016/j.fsi.2023.109084

	ROS-induced moderate autophagy of haemocytes confers resistance of Mercenaria mercenaria to air exposure stress
	Zhou, Cong1,2,3,4,5,6 ; Zou, Yan 7; Hu, Zhi 1,2,3,4,5,6; Yang, Mei-jie 1,2,3,4,6; Shi, Pu1,2,3,4,5,6 ; Li, Yong-ren 8; Guo, Yong-jun 8; Zhang, Tao1,2,3,4,6,9 ; Song, Hao 1,2,3,4,6,9
	2023-10-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	141 页码:11
通讯作者	Zhang, Tao([email protected]) ; Song, Hao([email protected])
摘要	Air exposure (AE) is a significant environmental stressor that can lead to desiccation, hypoxia, starvation, and disruption of cellular homeostasis in marine bivalves. Autophagy is a highly conserved catabolic pathway that facilitates the degradation of damaged macromolecules and organelles, thereby supporting cellular stress responses. To date, autophagy-mediated resistance mechanisms to AE stress remain largely elusive in bivalves. In this study, we performed a multi-tool approach to investigate the autophagy-related physiological regulation in hard clams (Mercenaria mercenaria) under different duration of AE (T = 0, 1, 5, 10, 20, 30 days). We observed that autophagy of haemocytes was significantly activated on day 5. However, autophagy activity began to significantly decline from day 10 to day 30. Autophagy was significantly inhibited after antioxidant treatment, indicating that reactive oxygen species (ROS) was an endogenous inducer of autophagy. A significant decline in the survival rate of hard clams was observed after injection of ammonium chloride or carbamazepine during AE stress, suggesting that moderate autophagy was conducive for clam survival under AE stress. We also observed DNA breaks and high levels of apoptosis in haemocytes on day 10. Activation of apoptosis lagged behind autophagy, and the relationship between autophagy and apoptosis might shift from antagonism to synergy with the duration of stress. This study provides novel insights into the stress resistance mechanisms in marine bivalves.
关键词	Air exposure Resistance Haemocytes Oxidative stress Autophagy
DOI	10.1016/j.fsi.2023.109084
收录类别	SCI
语种	英语
资助项目	Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao Pilot National Laboratory for Marine Science and Technology[2021AB34014]; Young Elite Scientists Sponsorship Program by CAST[LMEES-CTSP-2018-1]; Youth Innovation Promotion Association CAS[2021QNRC001]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:001101017000001
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS关键词	OXIDATIVE STRESS ; MYTILUS-GALLOPROVINCIALIS ; LYSOSOMAL REACTIONS ; CELL-DEATH ; APOPTOSIS ; RESPONSES ; IDENTIFICATION ; TEMPERATURE ; MECHANISMS ; PARAMETERS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183895
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Tao; Song, Hao
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Marine Sci Res Inst Shandong Prov, Qingdao 266100, Peoples R China 8.Tianjin Agr Univ, Fisheries Coll, Tianjin Key Lab Aquaecol & Aquaculture, Tianjin 300384, Peoples R China 9.7 Nanhai Rd, Qingdao City, Shandong, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Cong,Zou, Yan,Hu, Zhi,et al. ROS-induced moderate autophagy of haemocytes confers resistance of Mercenaria mercenaria to air exposure stress[J]. FISH & SHELLFISH IMMUNOLOGY,2023,141:11.
APA	Zhou, Cong.,Zou, Yan.,Hu, Zhi.,Yang, Mei-jie.,Shi, Pu.,...&Song, Hao.(2023).ROS-induced moderate autophagy of haemocytes confers resistance of Mercenaria mercenaria to air exposure stress.FISH & SHELLFISH IMMUNOLOGY,141,11.
MLA	Zhou, Cong,et al."ROS-induced moderate autophagy of haemocytes confers resistance of Mercenaria mercenaria to air exposure stress".FISH & SHELLFISH IMMUNOLOGY 141(2023):11.

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