Novel insights into toxin changes associated with the growth of<i> Alexandrium</i><i> pacificum:</i> Revealing active toxin-secretion ability and toxin cell quota variation

doi:10.1016/j.hal.2023.102516

	Novel insights into toxin changes associated with the growth of Alexandrium pacificum: Revealing active toxin-secretion ability and toxin cell quota variation
	Song, Weijia 1,2,3,4; Song, Xiuxian1,2,3,4 ; Chi, Lianbao 1,2,4; Zhu, Jianan 1,2,4; Cao, Xihua1,2,3,4 ; Yu, Zhiming1,2,3,4
	2023-11-01
发表期刊	HARMFUL ALGAE
ISSN	1568-9883
卷号	129 页码:8
通讯作者	Song, Xiuxian([email protected])
摘要	Paralytic shellfish toxins (PSTs) are widely distributed globally and are produced by Alexandrium pacificum in marine system. However, the characteristics of toxins producing and secreting associated with growth phases are still unclear, especially whether A. pacificum has the ability to actively secrete PSTs is controversial. In this study, variation characteristics of intracellular and extracellular PSTs contents associated with A. pacificum growth phases were investigated thoroughly. The results showed that intracellular and extracellular PSTs contents increased sharply during the exponential phase. But during the stationary phase, the intracellular PSTs content increased by only 26 %, and the extracellular PSTs content did not increase significantly. Since the increase in extracellular PSTs content mainly occurred at the exponential phase, when most cells were living, we speculated that active PSTs secretion of living cells might be an important production pathway of extracellular toxins besides leakage from dead cells. Furthermore, toxin cell quota variation associated with the growth phase was analysed. In the exponential phase, the toxin cell quota first increased and then decreased, with a maximum of 19.02 +/- 1.80 fmol/cell at 6 d. However, after entering the stationary phase, this value slowly increased again, suggesting that vigilance should be raised for the plateau of Alexandrium blooms. In addition, cells in the exponential phase mainly produced O-sulfated components such as GTX1&4, cells in the stationary phase mainly produced O-sulfate-free components such as GTX5. In this study, the toxigenic rules of A. pacificum were comprehensively uncovered, which provided theoretical guidance for the prevention and mitigation of A. pacificum blooms.
关键词	Alexandrium pacificum Extracellular toxins Toxin cell quota Growth phases
DOI	10.1016/j.hal.2023.102516
收录类别	SCI
语种	英语
资助项目	Intergovernmental Innovation Coop-eration Project of the Ministry of Science and Technology[2022YFE0136400]; National Natural Science Foundation of China[41976148]; National Natural Science Foundation of China[42276220]
WOS研究方向	Marine & Freshwater Biology
WOS类目	Marine & Freshwater Biology
WOS记录号	WOS:001097652800001
出版者	ELSEVIER
WOS关键词	SAXITOXIN ; DINOFLAGELLATE ; TEMPERATURE ; CULTURES ; CYANOBACTERIA ; BIOSYNTHESIS ; PURIFICATION ; DINOPHYCEAE ; KINETICS
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183835
专题	海洋生态与环境科学重点实验室
通讯作者	Song, Xiuxian
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Song, Weijia,Song, Xiuxian,Chi, Lianbao,et al. Novel insights into toxin changes associated with the growth of Alexandrium pacificum: Revealing active toxin-secretion ability and toxin cell quota variation[J]. HARMFUL ALGAE,2023,129:8.
APA	Song, Weijia,Song, Xiuxian,Chi, Lianbao,Zhu, Jianan,Cao, Xihua,&Yu, Zhiming.(2023).Novel insights into toxin changes associated with the growth of Alexandrium pacificum: Revealing active toxin-secretion ability and toxin cell quota variation.HARMFUL ALGAE,129,8.
MLA	Song, Weijia,et al."Novel insights into toxin changes associated with the growth of Alexandrium pacificum: Revealing active toxin-secretion ability and toxin cell quota variation".HARMFUL ALGAE 129(2023):8.

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