Effect of heat and hypoxia stress on mitochondrion and energy metabolism in the gill of hard clam

doi:10.1016/j.cbpc.2023.109556

	Effect of heat and hypoxia stress on mitochondrion and energy metabolism in the gill of hard clam
	Hu, Zhi 1,2,3,4,5,6,7; Xu, Li 5,8; Song, Hao 2,3,4,5,7; Feng, Jie 1,2,3,4,5,7; Zhou, Cong1,2,3,4,5,6 ; Yang, Mei-Jie 1,2,3,4,5,7; Shi, Pu1,2,3,4,5,6,7 ; Li, Yong-Ren 9; Guo, Yong-Jun 9; Li, Hai-Zhou 1; Zhang, Tao1,2,3,4,5,7
	2023-04-01
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
ISSN	1532-0456
卷号	266 页码:9
通讯作者	Zhang, Tao([email protected])
摘要	Aquatic animals suffer from heat and hypoxia stress more frequently due to global climate change and other anthropogenic activities. Heat and hypoxia stress can significantly affect mitochondrial function and energy metabolism. Here, the response and adaptation characteristics of mitochondria and energy metabolism in the gill of the hard clam Mercenaria mercenaria under heat (35 degrees C), hypoxia (0.2 mg/L), and heat plus hypoxia stress (35 degrees C, 0.2 mg/L) after 48 h exposure were investigated. Mitochondrial membrane potentials were depolarized under environmental stress. Mitochondrial fusion, fission and mitophagy played a key role in maintain mito-chondrion function. The AMPK subunits showed different expression under environmental stress. Acceleration of enzyme activities (phosphofructokinase, pyruvate kinase and lactic dehydrogenase) and accumulation of anaerobic metabolites in glycolysis and TCA cycle implied that the anaerobic metabolism might play a key role in providing energy. Accumulation of amino acids might help to increase tolerance under heat and heat combined hypoxia stress. In addition, urea cycle played a key role in amino acid metabolism to prevent ammonia/nitrogen toxicity. This study improved our understanding of the mitochondrial and energy metabolism responses of marine bivalves exposed to environmental stress.
关键词	Mercenaria mercenaria Heat Hypoxia Mitochondrion Energy metabolism
DOI	10.1016/j.cbpc.2023.109556
收录类别	SCI
语种	英语
资助项目	Tianjin science and technology commission project[20YFZCSN00240]; Tianjin Agricultural Committee Project[202103010]; Tianjin Agricultural Committee Project[SF1405303301]; Shandong Province Agricultural Major Applied Technology Innovation Project; Taishan Industrial Leading Talents Project[LMEES-CTSP-2018-1]; Double Hundred' Blue Industry Leader Team of Yantai; Creative Team Project of the Laboratory for Marine Ecology and Environ-mental Science, Qingdao Pilot National Laboratory for Marine Science and Technology[2021QNRC001]; Young Elite Scientists Sponsorship Program by CAST[CARS-49]; [LJNY201704]
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS记录号	WOS:000995226300001
出版者	ELSEVIER SCIENCE INC
WOS关键词	KEY SENSOR ; AMPK ; TEMPERATURE ; EXPRESSION ; RESPONSES ; OYSTER ; OXYGEN
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183063
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Tao
作者单位	1.Inst Oceanol, Chinese Acad Sci, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Lab Marine Ecol & Environm Sci, Qingdao Natl Lab Marine Sci & Technol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China 8.Tianjin Agr Univ, Fisheries Coll, Tianjin Key Lab Aqua ecol & Aquaculture, Tianjin 300384, Peoples R China 9.Shandong Fu Han Ocean Scitech Co Ltd, Haiyang 265100, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Hu, Zhi,Xu, Li,Song, Hao,et al. Effect of heat and hypoxia stress on mitochondrion and energy metabolism in the gill of hard clam[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2023,266:9.
APA	Hu, Zhi.,Xu, Li.,Song, Hao.,Feng, Jie.,Zhou, Cong.,...&Zhang, Tao.(2023).Effect of heat and hypoxia stress on mitochondrion and energy metabolism in the gill of hard clam.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,266,9.
MLA	Hu, Zhi,et al."Effect of heat and hypoxia stress on mitochondrion and energy metabolism in the gill of hard clam".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 266(2023):9.