Photoreversible DNA nanoswitch-based eluent-free strategy for the direct and effective isolation of highly-active thrombin from whole blood

doi:10.1016/j.ijbiomac.2023.124359

	Photoreversible DNA nanoswitch-based eluent-free strategy for the direct and effective isolation of highly-active thrombin from whole blood
	Lin, Yanna 1; Xu, Yulong 1; Xing, Yanzhi 1; Liu, Nazhen 2; Chen, Xuwei 1
	2023-06-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	239 页码:11
通讯作者	Liu, Nazhen([email protected]) ; Chen, Xuwei([email protected])
摘要	This study proposes an eluent-free isolation strategy for the direct isolation of thrombin from whole blood via tandem temperature/pH dual-responsive polyether sulfone monolith and photoreversible DNA nanoswitchfunctionalized metal-organic framework (MOF) aerogel. Temperature/pH dual-responsive microgel immobilized on polyether sulfone monolith was adopted to remove the matrix complexity of blood sample via size/ charge screening effect. Photoreversible DNA nanoswitches, comprising thrombin aptamer, aptamer complementary ssDNA (cDNA) and the azobenzene-modified ssDNA (control DNA), were functionalized on MOF aerogel to offer efficient capturing of thrombin under irradiation of ultraviolet light (365 nm), driven by electrostatic and hydrogen bond interactions. The release of captured thrombin was easily achieved by changing the complementary behaviors of DNA strands via blue light (450 nm) irradiation. Thrombin with purity higher than 95 % can be directly obtained from whole blood using this tandem isolation procedure. Fibrin production and substrate chromogenic tests showed that the released thrombin possessed high biological activity. The photoreversible thrombin capturing-release strategy is merited with eluent-free, avoiding the loss of activity of thrombin in chemical circumstances and undesired dilution, providing a robust guarantee for subsequent application.
关键词	Size charge screening Photoresponse DNA nanoswitch Eluent-free Thrombin
DOI	10.1016/j.ijbiomac.2023.124359
收录类别	SCI
语种	英语
资助项目	LiaoNing Science and Technology Development Foundation Guided by Central Government[2022JH6/100100024]; Fundamental Research Funds for the Cen-tral Universities[N2005027]; National Natural Science Foundation of China[22074011]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:000983807400001
出版者	ELSEVIER
WOS关键词	REVERSIBLE REGULATION ; PROTEIN INTERACTIONS ; G-QUADRUPLEX ; DEPLETION ; ABUNDANT ; APTAMER ; ADSORPTION ; SEPARATION ; STABILITY
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183009
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Liu, Nazhen; Chen, Xuwei
作者单位	1.Northeastern Univ, Coll Sci, Res Ctr Analyt Sci, Dept Chem, Box 332, Shenyang 110819, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Lin, Yanna,Xu, Yulong,Xing, Yanzhi,et al. Photoreversible DNA nanoswitch-based eluent-free strategy for the direct and effective isolation of highly-active thrombin from whole blood[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2023,239:11.
APA	Lin, Yanna,Xu, Yulong,Xing, Yanzhi,Liu, Nazhen,&Chen, Xuwei.(2023).Photoreversible DNA nanoswitch-based eluent-free strategy for the direct and effective isolation of highly-active thrombin from whole blood.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,239,11.
MLA	Lin, Yanna,et al."Photoreversible DNA nanoswitch-based eluent-free strategy for the direct and effective isolation of highly-active thrombin from whole blood".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 239(2023):11.