LysM-containing proteins function in the resistance of Litopenaeus vannamei against Vibrio parahaemolyticus infection

doi:10.1016/j.dci.2023.104900

IOCAS-IR > 实验海洋生物学重点实验室

	LysM-containing proteins function in the resistance of Litopenaeus vannamei against Vibrio parahaemolyticus infection
	Miao, Miao1,2,3 ; Li, Shihao 1,2,4; Yu, Yang1,2,4 ; Li, Fuhua 1,2,4,5
	2023-11-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	148 页码:9
通讯作者	Li, Shihao([email protected]) ; Li, Fuhua([email protected])
摘要	Lysin motif (LysM) is a functional domain that can bind to peptidoglycans, chitin and their derivatives. The LysM-containing proteins participate in multiple biological processes, such as the hydrolysis of bacterial cell walls and the perception of PAMPs in plants and high animals. In the present study, two genes encoding LysM-containing proteins, designated as LvLysM1 and LvLysM2, were identified in the Pacific white shrimp, Litopenaeus vannamei, and their functions during Vibrio infection were analyzed. The open-reading frame (ORF) of LvLysM1 was 795 bp, only encoding a LysM domain at the N-terminal region. The ORF of LvLysM2 was 834 bp, encoding a LysM domain at the central region and a transmembrane region at the C-terminal region. Both LvLysM1 and LvLysM2 were widely transcribed in all tested shrimp tissues. Enzyme-linked immunosorbent assay (ELISA) showed that the recombinant protein of LvLysM2 could bind to different bacterial polysaccharides, while LvLysM1 showed no direct binding activity. The transcripts of LvLysMs in gills increased significantly after infection with Vibrio parahaemolyticus. When LvLysM1 or LvLysM2 was knocked down by dsRNA, the mortality of shrimp was significantly increased after infection with Vibrio parahaemolyticus. Interestingly, some SNPs existed in these two genes were apparently correlated with the VpAHPND resistance of shrimp. These results suggested that LvLysM1 and LvLysM2 might contribute to the disease resistance of shrimp. The data provide new knowledge about the function of LysM-containing proteins in shrimp and potential genetic markers for disease resistance breeding.
关键词	Lysin motif Litopenaeus vannamei Binding activity V. parahaemolyticus SNPs
DOI	10.1016/j.dci.2023.104900
收录类别	SCI
语种	英语
资助项目	Key Program of National Natural Science Foundation of China[31972829]; General Program of National Natural Science Foundation of China; earmarked fund for CARS-48; Taishan Scholars Program; [31830100]
WOS研究方向	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS类目	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS记录号	WOS:001058518700001
出版者	ELSEVIER SCI LTD
WOS关键词	MOTIF (LYSM)-CONTAINING PROTEIN ; PEPTIDOGLYCAN-BINDING DOMAIN ; ANTIBACTERIAL RESPONSES ; AEROMONAS-HYDROPHILA ; NECROSIS DISEASE ; SHRIMP ; GENE ; EXPRESSION ; ASSOCIATION ; CLONING
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182874
专题	实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Miao, Miao,Li, Shihao,Yu, Yang,et al. LysM-containing proteins function in the resistance of Litopenaeus vannamei against Vibrio parahaemolyticus infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2023,148:9.
APA	Miao, Miao,Li, Shihao,Yu, Yang,&Li, Fuhua.(2023).LysM-containing proteins function in the resistance of Litopenaeus vannamei against Vibrio parahaemolyticus infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,148,9.
MLA	Miao, Miao,et al."LysM-containing proteins function in the resistance of Litopenaeus vannamei against Vibrio parahaemolyticus infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 148(2023):9.

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