Mechanisms of nucleic acid degradation and high hydrostatic pressure tolerance of a novel deep-sea wall-less bacterium

doi:10.1128/mbio.00958-23

IOCAS-IR > 实验海洋生物学重点实验室

	Mechanisms of nucleic acid degradation and high hydrostatic pressure tolerance of a novel deep-sea wall-less bacterium
	Zheng, Rikuan1,2,3,4 ; Wang, Chong1,2,3,4 ; Cai, Ruining 1,2,3,4,5; Shan, Yeqi 1,2,3,4,5; Sun, Chaomin 1,2,3,4,5
	2023-08-08
发表期刊	MBIO
ISSN	2150-7511
页码	19
通讯作者	Sun, Chaomin([email protected])
摘要	Wall-less bacteria are broadly distributed in diverse habitats. They evolved from a common ancestor within the Firmicutes phylum through reductive evolution. Here, we report the cultivation, characterization, and polyphasic taxonomic analysis of the novel free-living wall-less bacterium, Hujiaoplasma nucleasis zrk29. We demonstrated that strain zrk29 had a strong ability to degrade DNA and RNA both under laboratory conditions and in the deep sea. We found that nucleic acids induced strain zrk29 to release chronic bacteriophages which supported strain zrk29 and other marine bacteria to metabolize nucleic acids without lysing host cells. We also showed that strain zrk29 tolerated high hydrostatic pressure via two pathways: (i) by transporting cations into its cells to increase intracellular osmotic pressure and (ii) by adjusting the unsaturated fatty acid chain content in its cell membrane phospholipids to increase cell membrane fluidity. This study extends our understanding of free-living wall-less bacteria and provides a useful model to explore the unique adaptation mechanisms of deep-sea microbes. IMPORTANCEThe unique physiology and survival strategies of the Tenericutes bacterium-a typical wall-less bacterium-have fascinated scientists and the public, especially in extreme deep-sea environments where there is high hydrostatic pressure (HHP) and limited availability of nutrients. Here, we have isolated a novel free-living Tenericutes strain from deep-sea sediment and have found that it metabolizes nucleic acids with the support of chronic bacteriophages. This Tenericutes strain tolerates HHP stress by increasing intracellular osmotic pressure and the unsaturated fatty acid chain content of phospholipids in its cell membrane. Our results provide insights into the unique physiology of deep-sea free-living Tenericutes bacteria and highlight the significant role that chronic bacteriophages play in assisting wall-less bacteria to adapt to harsh conditions. The unique physiology and survival strategies of the Tenericutes bacterium-a typical wall-less bacterium-have fascinated scientists and the public, especially in extreme deep-sea environments where there is high hydrostatic pressure (HHP) and limited availability of nutrients. Here, we have isolated a novel free-living Tenericutes strain from deep-sea sediment and have found that it metabolizes nucleic acids with the support of chronic bacteriophages. This Tenericutes strain tolerates HHP stress by increasing intracellular osmotic pressure and the unsaturated fatty acid chain content of phospholipids in its cell membrane. Our results provide insights into the unique physiology of deep-sea free-living Tenericutes bacteria and highlight the significant role that chronic bacteriophages play in assisting wall-less bacteria to adapt to harsh conditions.
关键词	deep sea wall-less bacteria nucleic acid degradation high pressure tolerance chronic bacteriophage
DOI	10.1128/mbio.00958-23
收录类别	SCI
语种	英语
资助项目	National Natural Technology Innovation Project[42206107]; MOST \| National Natural Science Foundation of China (NSFC)[92051107]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:001044090300001
出版者	AMER SOC MICROBIOLOGY
WOS关键词	EXTRACELLULAR DNA ; MOLECULAR-BIOLOGY ; GENOMICS ; 2FE-2S ; IMPACT
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182712
专题	实验海洋生物学重点实验室深海极端环境与生命过程研究中心
通讯作者	Sun, Chaomin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Shandong, Peoples R China 5.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所; 深海极端环境与生命过程研究中心
通讯作者单位	中国科学院海洋研究所; 深海极端环境与生命过程研究中心
推荐引用方式 GB/T 7714	Zheng, Rikuan,Wang, Chong,Cai, Ruining,et al. Mechanisms of nucleic acid degradation and high hydrostatic pressure tolerance of a novel deep-sea wall-less bacterium[J]. MBIO,2023:19.
APA	Zheng, Rikuan,Wang, Chong,Cai, Ruining,Shan, Yeqi,&Sun, Chaomin.(2023).Mechanisms of nucleic acid degradation and high hydrostatic pressure tolerance of a novel deep-sea wall-less bacterium.MBIO,19.
MLA	Zheng, Rikuan,et al."Mechanisms of nucleic acid degradation and high hydrostatic pressure tolerance of a novel deep-sea wall-less bacterium".MBIO (2023):19.