Nitrogen and sulfur cycling driven by Campylobacterota in the sediment-water interface of deep-sea cold seep: a case in the South China Sea

doi:10.1128/mbio.00117-23

IOCAS-IR > 实验海洋生物学重点实验室

	Nitrogen and sulfur cycling driven by Campylobacterota in the sediment-water interface of deep-sea cold seep: a case in the South China Sea
	Sun, Qing-lei1,2,3 ; Xu, Ke 1,2; Cao, Lei4 ; Du, Zengfeng 4,5; Wang, Minxiao 4,6; Sun, Li 2,3,7
	2023-07-06
发表期刊	MBIO
ISSN	2150-7511
页码	17
通讯作者	Sun, Qing-lei([email protected]) ; Sun, Li([email protected])
摘要	Chemoautotrophs within Campylobacterota, especially Sulfurovum and Sulfurimonas, are abundant in the seawater-sediment interface of the Formosa cold seep in the South China Sea. However, the in situ activity and function of Campylobacterota are unknown. In this study, the geochemical role of Campylobacterota in the Formosa cold seep was investigated with multiple means. Two members of Sulfurovum and Sulfurimonas were isolated for the first time from deep-sea cold seep. These isolates are new chemoautotrophic species that can use molecular hydrogen as an energy source and CO2 as a sole carbon source. Comparative genomics identified an important hydrogen-oxidizing cluster in Sulfurovum and Sulfurimonas. Metatranscriptomic analysis detected high expression of hydrogen-oxidizing gene in the RS, suggesting that H-2 was likely an energy source in the cold seep. Genomic analysis indicated that the Sulfurovum and Sulfurimonas isolates possess a truncated sulfur-oxidizing system, and metatranscriptomic analysis revealed that Sulfurovum and Sulfurimonas with this genotype were active in the surface of RS and likely contributed to thiosulfate production. Furthermore, geochemical and in situ analyses revealed sharply decreased nitrate concentration in the sediment-water interface due to microbial consumption. Consistently, the denitrification genes of Sulfurimonas and Sulfurovum were highly expressed, suggesting an important contribution of these bacteria to nitrogen cycling. Overall, this study demonstrated that Campylobacterota played a significant role in the cycling of nitrogen and sulfur in a deep-sea cold seep. IMPORTANCEChemoautotrophs within Campylobacterota, in particular Sulfurovum and Sulfurimonas, are ubiquitous in deep-sea cold seeps and hydrothermal vents. However, to date, no Sulfurovum or Sulfurimonas has been isolated from cold seeps, and the ecological roles of these bacteria in cold seeps remain to be investigated. In this study, we obtained two isolates of Sulfurovum and Sulfurimonas from Formosa cold seep, South China Sea. Comparative genomics, metatranscriptomics, geochemical analysis, and in situ experimental study indicated collectively that Campylobacterota played a significant part in nitrogen and sulfur cycling in cold seep and was the cause of thiosulfate accumulation and sharp reduction of nitrate level in the sediment-water interface. The findings of this study promoted our understanding of the in situ function and ecological role of deep-sea Campylobacterota. Chemoautotrophs within Campylobacterota, in particular Sulfurovum and Sulfurimonas, are ubiquitous in deep-sea cold seeps and hydrothermal vents. However, to date, no Sulfurovum or Sulfurimonas has been isolated from cold seeps, and the ecological roles of these bacteria in cold seeps remain to be investigated. In this study, we obtained two isolates of Sulfurovum and Sulfurimonas from Formosa cold seep, South China Sea. Comparative genomics, metatranscriptomics, geochemical analysis, and in situ experimental study indicated collectively that Campylobacterota played a significant part in nitrogen and sulfur cycling in cold seep and was the cause of thiosulfate accumulation and sharp reduction of nitrate level in the sediment-water interface. The findings of this study promoted our understanding of the in situ function and ecological role of deep-sea Campylobacterota.
关键词	cold seep Sulfurimonas Sulfurovum Campylobacterota sulfur cycle nitrogen cycle
DOI	10.1128/mbio.00117-23
收录类别	SCI
语种	英语
资助项目	Science and Technology Innovation Project of Laoshan Laboratory[LSKJ202203103]; Innovation Research Group Project of the National Natural Science Foundation of China[42221005]; MOST \| National Natural Science Foundation of China (NSFC)[42030407]; MOST \| National Key Research and Development Program of China (NKPs)[2018YFC0310801]; Taishan Scholar Project of Shandong Province~
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:001026295200001
出版者	AMER SOC MICROBIOLOGY
WOS关键词	SP-NOV. ; EMENDED DESCRIPTION ; OXIDIZING CHEMOLITHOAUTOTROPH ; HYDROGEN ; BACTERIA
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182653
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室海洋地质与环境重点实验室
通讯作者	Sun, Qing-lei; Sun, Li
作者单位	1.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Laoshan Lab, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Deep Sea Res Ctr, Qingdao, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Marine Geol & Environm, Qingdao, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 7.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, Qing-lei,Xu, Ke,Cao, Lei,et al. Nitrogen and sulfur cycling driven by Campylobacterota in the sediment-water interface of deep-sea cold seep: a case in the South China Sea[J]. MBIO,2023:17.
APA	Sun, Qing-lei,Xu, Ke,Cao, Lei,Du, Zengfeng,Wang, Minxiao,&Sun, Li.(2023).Nitrogen and sulfur cycling driven by Campylobacterota in the sediment-water interface of deep-sea cold seep: a case in the South China Sea.MBIO,17.
MLA	Sun, Qing-lei,et al."Nitrogen and sulfur cycling driven by Campylobacterota in the sediment-water interface of deep-sea cold seep: a case in the South China Sea".MBIO (2023):17.