Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)

doi:10.3390/ijms241411770

IOCAS-IR > 实验海洋生物学重点实验室

	Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)
	Wang, Xueying1,2 ; Zhao, Ning 1,2,3; Wang, Tao 1,2,4; Huang, Jinwei 1,2,3; Liu, Qinghua 1,2; Li, Jun 1,2
	2023-07-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1661-6596
卷号	24 期号:14 页码:12
通讯作者	Liu, Qinghua([email protected]) ; Li, Jun([email protected])
摘要	The development and maturation of sperm entails intricate metabolic processes involving water molecules, amino acids, hormones, and various substances. Among these processes, the role of aquaporins (aqps) in the testis is crucial. Turbot (Scophthalmus maximus) is a significant marine flatfish species in China; however, natural egg laying in females is not feasible under cultured conditions. Consequently, artificial insemination becomes necessary, requiring the retrieval of sperm and eggs through artificial methods. In this study, we combined genomic, transcriptomics, RT-qPCR, computer-assisted sperm analysis (CASA), and immunohistochemistry to investigate the involvement of the aqp family in spermatogenesis in turbot. Through genomic data analysis, we identified 16 aqps genes dispersed across 13 chromosomes, each exhibiting the characteristic major intrinsic protein (MIP) domain associated with AQPs. The results from RNA-seq and RT-qPCR analysis revealed prominent expression of aqp4, 10, and 12 during the proliferative stage, whereas aqp1 showed primary expression during the mature stage. aqp11 displayed high expression levels during both MSII and MSV stages, potentially contributing significantly to the proliferation and maturation of male germ cells. Conversely, aqp8 showed elevated expression levels during the MSIII, MSIII-IV, and MSIV stages, suggesting its direct involvement in spermiogenesis. Immunohistochemical analysis unveiled the predominant localization of AQP1 protein in male germ cells rather than Sertoli cells, specifically concentrated in the head of sperm within cysts. Furthermore, a noteworthy decline in sperm motility was observed when sperm were subjected to treatment with either the AQP1-specific inhibitor (HgCl2) or the AQP1 antibody. However, no direct correlation was found between the expression of Smaqp1 and sperm quality. Overall, these findings provide new insights into the involvement of aqps in teleost spermatogenesis. Moreover, they hold potential for improving techniques related to sperm activation and cryopreservation, offering valuable knowledge for future advancements in this field.
关键词	turbot aqps spermatogenesis sperm
DOI	10.3390/ijms241411770
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:001036142300001
出版者	MDPI
WOS关键词	PHYSIOLOGICAL VOLUME REGULATION ; SPERM MOTILITY ; EXPRESSION ; CHANNELS ; AQUAPORINS ; TESTIS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182575
专题	实验海洋生物学重点实验室
通讯作者	Liu, Qinghua; Li, Jun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xueying,Zhao, Ning,Wang, Tao,et al. Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2023,24(14):12.
APA	Wang, Xueying,Zhao, Ning,Wang, Tao,Huang, Jinwei,Liu, Qinghua,&Li, Jun.(2023).Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,24(14),12.
MLA	Wang, Xueying,et al."Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24.14(2023):12.