Engineering an antifouling electrochemical aptasensor based on a designed zwitterionic peptide for tetracycline detection in milk

doi:10.1016/j.foodcont.2023.109929

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	Engineering an antifouling electrochemical aptasensor based on a designed zwitterionic peptide for tetracycline detection in milk
	Li, Yonghui 1; Pu, Xujun 1; Niu, Meirong 1; Fan, Xuejing 1; Ding, Yangyue 1; Ma, Wenlong2 ; Gu, Ying 1
	2023-11-01
发表期刊	FOOD CONTROL
ISSN	0956-7135
卷号	153 页码:12
通讯作者	Ma, Wenlong([email protected]) ; Gu, Ying([email protected])
摘要	Interference derived from nonspecific attachment of foulants (e.g. proteins) from food matrices (fouling) on sensor surfaces has always been a major issue in food safety sensing, deteriorating the stability and accuracy of detection. Engineering and introducing antifouling materials on sensors to strongly hydrate their surfaces is a promising approach to address this issue. Here, inspired by a bioactive peptide with antithrombotic function, a novel antifouling zwitterionic peptide (Z-peptide) with a sequence of NH2-CPPPPEKDQDK (C & PRIME;- N') was designed. The antifouling performance of the proposed Z-peptides modified sensor surface was demonstrated in different protein solutions and milk samples (signal suppression rate as low as 1.87%). Molecular dynamics (MD) simulations reveal that a large number of hydrogen bonds and strong interaction forces are formed between Z-peptides and water molecules, contributing to the formation of a dense hydration layer on the sensor surface to resist nonspecific adsorption. An electrochemical aptasensor based on the Z-peptide was developed for tetracycline (TC) detection. The proposed sensor was able to detect TC with a wide linear range (0.01-100 ng mL-1) and a low limit of detection (LOD = 2.91 pg mL-1, S/N ﹦3) and showed favorable specificity and stability. Given the excellent resistance to nonspecific adsorption, the antifouling sensor was capable of assaying TC in milk by diluting without other pretreatments (recoveries: 107.17%-110.16%).
关键词	Antifouling Zwitterionic peptide Electrochemical aptasensor Tetracycline Food safety
DOI	10.1016/j.foodcont.2023.109929
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[32102073]; Yunnan Fundamental Research Projects[202201AU070106]; Yunnan Fundamental Research Projects[202101BE070001-052]; Yunnan Science and Technology Planning Project[202102AE090021]; Yunnan Major Scientific and Technological Projects[202202AG050009]
WOS研究方向	Food Science & Technology
WOS类目	Food Science & Technology
WOS记录号	WOS:001020388400001
出版者	ELSEVIER SCI LTD
WOS关键词	PERFORMANCE
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182395
专题	中国科学院海洋研究所
通讯作者	Ma, Wenlong; Gu, Ying
作者单位	1.Kunming Univ Sci & Technol, Fac Food Sci & Engn, Kunming 650500, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Yonghui,Pu, Xujun,Niu, Meirong,et al. Engineering an antifouling electrochemical aptasensor based on a designed zwitterionic peptide for tetracycline detection in milk[J]. FOOD CONTROL,2023,153:12.
APA	Li, Yonghui.,Pu, Xujun.,Niu, Meirong.,Fan, Xuejing.,Ding, Yangyue.,...&Gu, Ying.(2023).Engineering an antifouling electrochemical aptasensor based on a designed zwitterionic peptide for tetracycline detection in milk.FOOD CONTROL,153,12.
MLA	Li, Yonghui,et al."Engineering an antifouling electrochemical aptasensor based on a designed zwitterionic peptide for tetracycline detection in milk".FOOD CONTROL 153(2023):12.

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