Muricauda okinawensis sp. Nov. and Muricauda yonaguniensis sp. Nov., Two Marine Bacteria Isolated from the Sediment Core near Hydrothermal Fields of Southern Okinawa Trough

doi:10.3390/microorganisms11061580

IOCAS-IR > 海洋地质与环境重点实验室

	Muricauda okinawensis sp. Nov. and Muricauda yonaguniensis sp. Nov., Two Marine Bacteria Isolated from the Sediment Core near Hydrothermal Fields of Southern Okinawa Trough
	Cao, Wenrui 1; Deng, Xingyu 1,2; Jiang, Mingyu 1; Zeng, Zhigang1 ; Chang, Fengming 1
	2023-06-01
发表期刊	MICROORGANISMS
卷号	11 期号:6 页码:16
通讯作者	Cao, Wenrui([email protected])
摘要	Two strains, 81s02(T) and 334s03(T), were isolated from the sediment core near the hydrothermal field of southern Okinawa Trough. The cells of both strains were observed to be rod-shaped, non-gliding, Gram-staining negative, yellow-pigmented, facultatively anaerobic, catalase and oxidase positive, and showing optimum growth at 30 & DEG;C and pH 7.5. The strains 81s02(T) and 334s03(T) were able to tolerate up to 10% and 9% (w/v) NaCl concentration, respectively. Based on phylogenomic analysis, the average nucleotide identity (ANI) and the digital DNA-DNA hybridization (dDDH) values between the two strains and the nearest phylogenetic neighbors of the genus Muricauda were in range of 78.0-86.3% and 21.5-33.9%, respectively. The strains 81s02(T) and 334s03(T) shared 98.1% 16S rRNA gene sequence similarity to each other but were identified as two distinct species based on 81.4-81.5% ANIb, 85.5-85.6% ANIm and 25.4% dDDH values calculated using whole genome sequences. The strains 81s02(T) and 334s03(T) shared the highest 16S rRNA gene sequence similarity to M. lutimaris SMK-108(T) (98.7%) and M. aurea BC31-1-A7(T) (98.8%), respectively. The major fatty acid of strains 81s02(T) and 334s03(T) were identified similarly as iso-C-15:0, iso-C-17:0 3-OH and iso-C-15:1 G, and the major polar lipids of the both strains consisted of phosphatidylethanolamine and two unidentified lipids. The strains contained MK-6 as their predominant menaquinone. The genomic G+C contents of strains 81s02(T) and 334s03(T) were determined to be 41.6 and 41.9 mol%, respectively. Based on the phylogenetic and phenotypic characteristics, both strains are considered to represent two novel species of the genus Muricauda, and the names Muricauda okinawensis sp. nov. and Muricauda yonaguniensis sp. nov. are proposed for strains 81s02(T) (=KCTC 92889(T) = MCCC 1K08502(T)) and 334s03(T) (=KCTC 92890(T) = MCCC 1K08503(T)).
关键词	Flavobacteriaceae Muricauda okinawensis sp nov Muricauda yonaguniensis sp polyphasic taxonomy hydrothermal field
DOI	10.3390/microorganisms11061580
收录类别	SCI
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:001014976100001
出版者	MDPI
WOS关键词	EMENDED DESCRIPTION ; FAMILY FLAVOBACTERIACEAE ; MICROBIAL COMMUNITIES ; IHEYA NORTH ; GEN. NOV. ; SEA ; SEAWATER ; DIVERSITY ; SEQUENCES ; DATABASE
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182328
专题	海洋地质与环境重点实验室
通讯作者	Cao, Wenrui
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China 2.Shandong Univ Sci & Technol, Coll Earth Sci & Engn, Qingdao 266590, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Cao, Wenrui,Deng, Xingyu,Jiang, Mingyu,et al. Muricauda okinawensis sp. Nov. and Muricauda yonaguniensis sp. Nov., Two Marine Bacteria Isolated from the Sediment Core near Hydrothermal Fields of Southern Okinawa Trough[J]. MICROORGANISMS,2023,11(6):16.
APA	Cao, Wenrui,Deng, Xingyu,Jiang, Mingyu,Zeng, Zhigang,&Chang, Fengming.(2023).Muricauda okinawensis sp. Nov. and Muricauda yonaguniensis sp. Nov., Two Marine Bacteria Isolated from the Sediment Core near Hydrothermal Fields of Southern Okinawa Trough.MICROORGANISMS,11(6),16.
MLA	Cao, Wenrui,et al."Muricauda okinawensis sp. Nov. and Muricauda yonaguniensis sp. Nov., Two Marine Bacteria Isolated from the Sediment Core near Hydrothermal Fields of Southern Okinawa Trough".MICROORGANISMS 11.6(2023):16.