Zebrafish (Danio rerio) TR beta- and TTR-based electrochemical biosensors: Construction and application for the evaluation of thyroid-disrupting activity of bisphenols

doi:10.1016/j.envpol.2023.121745

IOCAS-IR > 实验海洋生物学重点实验室

	Zebrafish (Danio rerio) TR beta- and TTR-based electrochemical biosensors: Construction and application for the evaluation of thyroid-disrupting activity of bisphenols
	Li, Yuejiao 1; Zhang, Zhenzhong 1; Wang, Jun 1; Shan, Yeqi 2,3,4; Tian, Hua 1; Cui, Pengfei 1; Ru, Shaoguo 1
	2023-08-01
发表期刊	ENVIRONMENTAL POLLUTION
ISSN	0269-7491
卷号	330 页码:9
通讯作者	Ru, Shaoguo([email protected])
摘要	Thyroid-disrupting chemicals (TDCs) have received increasing concerns because of their negative health impacts on both wildlife and humans. This study aimed to develop in vitro screening assays for TDCs based on thyroid hormone receptor beta (TR beta) and transthyretin (TTR) proteins. Firstly, the recombinant ligand-binding domain of TR beta (TR beta-LBD) and TTR proteins of zebrafish were produced by eukaryotic expression system and then used as bio-recognition components to construct electrochemical biosensors. In the biosensors, the supported bilayer lipid membrane (s-BLM) was used as a matrix to immobilize proteins, and gold nanoflowers (AuNFs) were used to improve the sensitivity by increasing electroactive surface area. Under the optimizing conditions, the zfTR beta-LBD/AuNFs/s-BLM/GCE biosensor had a detection range of 0.23 nM-1.92 mu M and a detection limit of 0.07 nM for triiodothyronine (T-3), while the zfTTR/AuNFs/s-BLM/GCE biosensor had a detection range of 0.46 nM-3.84 mu M, with a detection limit of 0.13 nM. Based on the constructed biosensors, the order of T-3 equivalent concentrations of bisphenols was BPA approximate to BPS > BPF > BPAF approximate to BPAP > BPZ, which was similar to the results of recombinant TR beta two-hybrid yeast assay. Furthermore, the reliability of the biosensors was validated by molecular docking, in which BPA and BPS showed higher binding affinity to zfTR beta-LBD. Therefore, this study provided a valuable tool for efficiently screening TDCs.
关键词	Thyroid-disrupting chemicals Thyroid hormone receptor beta Transthyretin In vitro screening Assay Electrochemical biosensor
DOI	10.1016/j.envpol.2023.121745
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41976139]; National Natural Science Foundation of China[21607144]
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000998593300001
出版者	ELSEVIER SCI LTD
WOS关键词	ESTROGENIC ACTIVITY ; IN-VITRO ; IDENTIFICATION ; CHEMICALS ; DRINKING ; PROTOCOL ; PROTEIN
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182256
专题	实验海洋生物学重点实验室
通讯作者	Ru, Shaoguo
作者单位	1.Ocean Univ China, Coll Marine Life Sci, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 3.Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Chinese Acad Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Yuejiao,Zhang, Zhenzhong,Wang, Jun,et al. Zebrafish (Danio rerio) TR beta- and TTR-based electrochemical biosensors: Construction and application for the evaluation of thyroid-disrupting activity of bisphenols[J]. ENVIRONMENTAL POLLUTION,2023,330:9.
APA	Li, Yuejiao.,Zhang, Zhenzhong.,Wang, Jun.,Shan, Yeqi.,Tian, Hua.,...&Ru, Shaoguo.(2023).Zebrafish (Danio rerio) TR beta- and TTR-based electrochemical biosensors: Construction and application for the evaluation of thyroid-disrupting activity of bisphenols.ENVIRONMENTAL POLLUTION,330,9.
MLA	Li, Yuejiao,et al."Zebrafish (Danio rerio) TR beta- and TTR-based electrochemical biosensors: Construction and application for the evaluation of thyroid-disrupting activity of bisphenols".ENVIRONMENTAL POLLUTION 330(2023):9.