Degradation of seaweed waste by the extracellular multifunctional Amy19 enzyme and the stimulatory effects of enzymatic hydrolysates on cucumber seed germination

doi:10.1007/s10811-023-03086

IOCAS-IR > 实验海洋生物学重点实验室

	Degradation of seaweed waste by the extracellular multifunctional Amy19 enzyme and the stimulatory effects of enzymatic hydrolysates on cucumber seed germination
	Zhao, Luying 1,2; Fu, Liping 1,2; Gu, Xiaoqian 3; Zhang, Qian 1,2; Li, Jiang 1,2
	2023-09-09
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	35 期号:6 页码:9
通讯作者	Li, Jiang([email protected])
摘要	In the present study, recombinant Escherichia coli that secretes the multifunctional.-amylase Amy19 was constructed and the induction conditions were optimized. The optimal induction condition obtained by orthogonal experiment was addition of 1.5% glycine for 24 h at 10(circle)C. The extracellular secreted multifunctional Amy19 showed the ability to degrade seaweed (Gracilaria verrucosa) waste directly. The reducing sugar concentration increased gradually with degradation time, reaching a maximum (1.047 +/- 0.0058 mg mL(- 1)) at 60 h. HPLC showed that the higher degree of polymerization of oligosaccharides decreased gradually, while the content of galactose and 3,6-anhydro-L-galactose gradually increased with time. In the cucumber seed germination experiments, root lengths and seed germination percentages after treatment with enzymatic hydrolysates of seaweed at 5% and 10% concentrations were much greater than after treatment with water or water extracts of seaweed. This may be due to the presence of polysaccharides and oligosaccharide in EHS. In addition, seeds treated with enzymatic hydrolysates of seaweed at lower concentrations showed higher germination indexes and germination energy levels, which were associated with lower mean germination times. Thus, the use of the lower concentrations of enzymatic hydrolysates of seaweed may result in more rapid germination of seeds. This study provides important information for the efficient preparation of recombinant enzyme extracellular secretion and the agricultural applications of seaweed waste.
关键词	Multifunctional alpha-amylase Amy19 Enzymatic hydrolysates Cucumber seed Germination Extracellular secretion
DOI	10.1007/s10811-023-03086
收录类别	SCI
语种	英语
资助项目	Impact and Response of Antarctic Seas to Climate Change[RFSOCC2020-2022]; National Key Research and Development Program of China[2018YFC1406704]; Key Research and Development Program of Shandong Province, China[2018GHY115013]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:001065090800002
出版者	SPRINGER
WOS关键词	PLANT-GROWTH REGULATORS ; KAPPA-CARRAGEENAN ; LAMBDA-CARRAGEENAN ; MOLECULAR-WEIGHT ; ACID-HYDROLYSIS ; FUNCTIONAL FOOD ; ULVA-LACTUCA ; MACROALGAE ; EXTRACTS ; METABOLITES
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181692
专题	实验海洋生物学重点实验室
通讯作者	Li, Jiang
作者单位	1.Minist Nat Resources, Inst Oceanog 1, Marine Bioresource & Environm Res Ctr, Qingdao 266061, Peoples R China 2.Minist Nat Resources, Inst Oceanog 1, Key Lab Ecol Environm Sci & Technol, Qingdao 266061, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Luying,Fu, Liping,Gu, Xiaoqian,et al. Degradation of seaweed waste by the extracellular multifunctional Amy19 enzyme and the stimulatory effects of enzymatic hydrolysates on cucumber seed germination[J]. JOURNAL OF APPLIED PHYCOLOGY,2023,35(6):9.
APA	Zhao, Luying,Fu, Liping,Gu, Xiaoqian,Zhang, Qian,&Li, Jiang.(2023).Degradation of seaweed waste by the extracellular multifunctional Amy19 enzyme and the stimulatory effects of enzymatic hydrolysates on cucumber seed germination.JOURNAL OF APPLIED PHYCOLOGY,35(6),9.
MLA	Zhao, Luying,et al."Degradation of seaweed waste by the extracellular multifunctional Amy19 enzyme and the stimulatory effects of enzymatic hydrolysates on cucumber seed germination".JOURNAL OF APPLIED PHYCOLOGY 35.6(2023):9.