Phenethylamine Is a Potential Density Stress Pheromone in Turbot (<i>Scophthalmus maximus</i>)

doi:10.3390/fishes8100506

IOCAS-IR > 实验海洋生物学重点实验室

	*Phenethylamine Is a Potential Density Stress Pheromone in Turbot (Scophthalmus maximus)*
	Li, Jiyuan1,2,3,4,5 ; Wang, Yanfeng 1,2,3,4; Guo, Teng 1,2,3,4,6,7; Xu, Shihong 1,2,3,4; Gao, Guang1,2,3,4 ; Liu, Feng 1,2,3,4,5; Guo, Xiaoyang 1,2,3,4,8; Wu, Yanduo 1,2,3,4,5; Zhao, Haixia1,2,3,4,5 ; Li, Jun 1,2,3,4
	2023-10-01
发表期刊	FISHES
卷号	8 期号:10 页码:17
通讯作者	Wang, Yanfeng([email protected]) ; Li, Jun([email protected])
摘要	Pheromones play a vital role in regulating fish behavior, including reproduction, aggregation, hazard recognition and food location. To gain a better understanding of chemical communication in fish produced by density changes, this study analyzed the metabolites released by turbot (Scophthalmus maximus) under different stocking densities. The experiment was conducted at low (LD: 3.01 kg/m(2)), medium (MD: 6.62 kg/m(2)) and high (HD: 10.84 kg/m(2)) densities for 15 days. High-throughput non-targeted metabolomics (LC-MS/MS) was used to identify variations in metabolites released into the aquatic environment by turbot at different densities. Results showed that 29 and 47 metabolites were significantly upregulated in the MD and HD groups, respectively, compared with the LD group. Among them, hexadecanedioic acid, xanthine, phenethylamine, proline and styrene were significantly upregulated in the MD vs. LD, HD vs. MD and HD vs. LD. The VIP diagram of OPLS-DA alignment showed that phenethylamine was the most important metabolite shared by MD vs. LD, HD vs. MD and HD vs. LD. Key gene changes in the GH/IGF-1 signaling pathway, HPI axis of turbot were studied using qRT-PCR for density treatment. The results demonstrated that the expression of GH, GHR and IGF-1 was significantly lower, while the expression of CRH and ACTH was higher in the HD group. Additionally, plasma levels of cortisol, glucose, triglycerides and T-3 were also highest in the HD group compared with the LD and MD groups. Phenylethylamine concentration was positively correlated with the HPI axis and negatively correlated with the GH/IGF-1 signaling pathway. To investigate the impact of phenethylamine accumulation on turbot, an acute treatment experiment with phenethylamine was set up. Its concentration in the aquatic environment was set at 0 (CON), 10(-7) (LP) and 10(-5) (HP) mol/L via exogenous addition, and turbot were exposed to these environments for 2 days. There was a high degree of concordance between the GH/IGF-1 signaling pathway (GH, GHR, IGF-1), HPI axis (CRH, ACTH) and plasma physiological changes (cortisol, glucose, triglycerides, T-3) in the phenethylamine-treated group and the density-treated group. Therefore, accumulation of phenethylamine with increasing stocking density may be a potential cause of density stress. Phenylethylamine has a dose-dependent and trace effect as a pheromone.
关键词	pheromone phenethylamine metabolomics Scophthalmus maximus stocking density
DOI	10.3390/fishes8100506
收录类别	SCI
语种	英语
资助项目	Special thanks to the China Weihai Institute of Marine Biotechnology (Zhe Liu, Xiaoyang Ma, Jialin Li, Jingqiang Yang) for their support.
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:001092469900001
出版者	MDPI
WOS关键词	XANTHINE-OXIDASE ACTIVITY ; D-AMINO ACIDS ; STOCKING DENSITY ; JUVENILE TURBOT ; PHYSIOLOGICAL-RESPONSE ; BETA-PHENYLETHYLAMINE ; GROWTH-PERFORMANCE ; ANTIOXIDANT STATUS ; IMMUNE-RESPONSE ; WATER-QUALITY
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181614
专题	实验海洋生物学重点实验室
通讯作者	Wang, Yanfeng; Li, Jun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 7.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Peoples R China 8.Qingdao Agr Univ, Sch Life Sci, Qingdao 266109, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Jiyuan,Wang, Yanfeng,Guo, Teng,et al. Phenethylamine Is a Potential Density Stress Pheromone in Turbot (Scophthalmus maximus)[J]. FISHES,2023,8(10):17.
APA	Li, Jiyuan.,Wang, Yanfeng.,Guo, Teng.,Xu, Shihong.,Gao, Guang.,...&Li, Jun.(2023).Phenethylamine Is a Potential Density Stress Pheromone in Turbot (Scophthalmus maximus).FISHES,8(10),17.
MLA	Li, Jiyuan,et al."Phenethylamine Is a Potential Density Stress Pheromone in Turbot (Scophthalmus maximus)".FISHES 8.10(2023):17.

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