Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements

doi:10.3390/ijms232315033

	Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements
	Li, Fengting1,2,3 ; Yue, Caixia 1,4; Deng, Yunyan 1,2,3; Tang, Ying Zhong 1,2,3
	2022-12-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	23 期号:23 页码:16
通讯作者	Deng, Yunyan([email protected]) ; Tang, Ying Zhong([email protected])
摘要	Similar to the seeds of higher plants, resting cysts, a non-motile, benthic, and dormant stage in the life history of many dinoflagellate species, play vital roles via germination in the seasonal dynamics and particularly the initiation of harmful algal blooms (HABs) of dinoflagellates. It is thus crucial for resting cysts to balance between the energetic catabolism for viability maintenance and the energy preservation for germination during their dormancy. Despite this importance, studies on how resting cysts of dinoflagellates accomplish energetic metabolism in marine sediment have been virtually absent. In this study, using the cosmopolitan HABs-causing species Scrippsiella acuminata as a representative, we measured the transcriptional activity of the most efficient pathway of the energy catabolism tricarboxylic acid (TCA) cycle, cell viability (via neutral red staining), and the cellular ATP content of resting cysts under a set of mock conditions in marine sediments (e.g., 4 degrees C, darkness, and anoxia) for a maximum period of one year. Based on the correlation analyses among the expression levels of genes, cyst viability, and ATP content, we revealed that the TCA cycle was still a crucial pathway of energetic catabolism for resting cysts under aerobic conditions, and its expression was elevated at higher temperatures, light irradiation, and the early stage of dormancy. Under anaerobic conditions, however, the TCA cycle pathway ceased expression in resting cysts, as also supported by ATP measurements. Our results have laid a cornerstone for the comprehensive revelation of the energetic metabolism and biochemical processes of dormancy of resting cysts in marine sediments.
关键词	ATP content energetic metabolism resting cyst Scrippsiella acuminata tricarboxylic acid cycle viability
DOI	10.3390/ijms232315033
收录类别	SCI
语种	英语
资助项目	Key Deployment Project of Centre for Ocean Mega - Research of Science, Chinese Academy of Sciences[COMS2019Q09]; National Science Foundation of China[42176207]; Marine Samp;T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2021QNLM040001]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000897362700001
出版者	MDPI
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181080
专题	海洋生态与环境科学重点实验室
通讯作者	Deng, Yunyan; Tang, Ying Zhong
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Fengting,Yue, Caixia,Deng, Yunyan,et al. Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(23):16.
APA	Li, Fengting,Yue, Caixia,Deng, Yunyan,&Tang, Ying Zhong.(2022).Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(23),16.
MLA	Li, Fengting,et al."Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.23(2022):16.

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