Exposure to nitrate induces alterations in blood parameter responses, liver immunity, and lipid metabolism in juvenile turbot (Scophthalmus maximus)

doi:10.1016/j.aquatox.2022.106280

IOCAS-IR > 实验海洋生物学重点实验室

	Exposure to nitrate induces alterations in blood parameter responses, liver immunity, and lipid metabolism in juvenile turbot (Scophthalmus maximus)
	Yu, Jiachen 1,3; Wang, Xingqiang 1,3; Qian, Shiyue 1; Liu, Pengfei 1; Li, Xian 4; Li, Jun 2
	2022-10-01
发表期刊	AQUATIC TOXICOLOGY
ISSN	0166-445X
卷号	251 页码:11
通讯作者	Li, Xian([email protected]) ; Li, Jun([email protected])
摘要	Nitrate (NO3-) pollution of waterbodies has attracted significant global attention as it poses a serious threat to aquatic organisms and human beings. This study aimed to evaluate the role of NO3-, an end product of biological nitrification processes, in immune status and lipid metabolism to have a comprehensive understanding of its toxic effects on fishes. Therefore, in this work, juvenile turbot (Scophthalmus maximus) were subjected to four nominal concentrations of NO3- (i.e., 0, 50, 200, 400 mg/L of NO3--N) for a 60-day period. The results indicated that increased exposure to NO3- (200 and/or 400 mg/L) enhanced the concentrations of plasma heat shock protein concentrations (HSP70), complement component 3 (C3), complement component 4 (C4), immunoglobulin M (IgM) and lysozyme (LYS), which meant that NO3- caused fluctuations in the plasma immune system. Higher exposure to NO3- (200 and/or 400 mg/L) also caused significant enhancements in plasma glutamic pyruvic transaminase (GPT), as well as glutamic oxaloacetic transaminase (GOT) activity. Furthermore, NO3- exposure resulted in upregulation of liver TNF-alpha, IL-1 beta, HSP70, HSP90, and LYS. Additionally, the results suggested that NO3- exposure caused a certain degree of histological damage and inflammation in the liver and activated the immune defense processes of juvenile turbot. Furthermore, the mRNA expression levels of certain genes associated with lipid metabolism (peroxisome proliferator-activated receptor-alpha [PPAR-alpha], carnitine palmitoyltransferase 1[CPT1], liver X receptor [LXR] together with sterol regulatory element binding protein-1 [SREBP-1]) increased significantly within fish liver exposed to 200/400 mg/L NO3- -N treatments. Finally, the results obtained from the analysis of the integrated biological responses version 2 (IBRv2) also confirmed the toxic effects of NO3- on juvenile turbot. According to these findings, it can be found that NO3- emission in the aquatic environment needs to be strictly controlled, as it may cause immune and lipid metabolism disorders in fish.
关键词	Nitrate exposure Plasma immune parameters Lipid metabolism IBRv2 Scophthalmus maximus
DOI	10.1016/j.aquatox.2022.106280
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program[2018YFD0901204]; China Agriculture Research System[CARS -47-G21]; Priority Academic Program Development of Jiangsu Higher Education Institutions[2021JSPAPD010]
WOS研究方向	Marine & Freshwater Biology ; Toxicology
WOS类目	Marine & Freshwater Biology ; Toxicology
WOS记录号	WOS:000853356800003
出版者	ELSEVIER
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180787
专题	实验海洋生物学重点实验室
通讯作者	Li, Xian; Li, Jun
作者单位	1.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Biotechnol, Lab Pathol & Immunol Aquat Anim, Lianyungang 222005, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222005, Peoples R China 4.Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yu, Jiachen,Wang, Xingqiang,Qian, Shiyue,et al. Exposure to nitrate induces alterations in blood parameter responses, liver immunity, and lipid metabolism in juvenile turbot (Scophthalmus maximus)[J]. AQUATIC TOXICOLOGY,2022,251:11.
APA	Yu, Jiachen,Wang, Xingqiang,Qian, Shiyue,Liu, Pengfei,Li, Xian,&Li, Jun.(2022).Exposure to nitrate induces alterations in blood parameter responses, liver immunity, and lipid metabolism in juvenile turbot (Scophthalmus maximus).AQUATIC TOXICOLOGY,251,11.
MLA	Yu, Jiachen,et al."Exposure to nitrate induces alterations in blood parameter responses, liver immunity, and lipid metabolism in juvenile turbot (Scophthalmus maximus)".AQUATIC TOXICOLOGY 251(2022):11.

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