Genome-Wide Identification of Laminin Family Related to Follicular Pseudoplacenta Development in Black Rockfish (Sebastes schlegelii)

doi:10.3390/ijms231810523

IOCAS-IR > 实验海洋生物学重点实验室

	Genome-Wide Identification of Laminin Family Related to Follicular Pseudoplacenta Development in Black Rockfish (Sebastes schlegelii)
	Zhao, Ning 1,2,3,4; Wang, Xueying1,2,3 ; Wang, Tao 1,2,3,5; Xu, Xiaojie1,2,3,4 ; Liu, Qinghua 1,2,3; Li, Jun 1,2,3
	2022-09-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	23 期号:18 页码:12
通讯作者	Liu, Qinghua([email protected]) ; Li, Jun([email protected])
摘要	As major elements of the basement membrane, laminins play a significant role in angiogenesis, migration, and adhesion of various cells. Sebastes schlegelii is a marine viviparous teleost of commercial importance. Previous research has reported abundant blood vessels and connective tissue in the ovary during gestation. In this study, 14 laminin genes of the alpha, beta, and gamma subfamilies from genomic data were identified based on zebrafish and human laminins, distributed on 9 chromosomes in S. schlegelii. Analysis of structural domains showed that coiled-coil regions and EGF domains existed in all laminin genes. Moreover, via qPCR, we found that the expression of laminin genes, including lama4, lama5, lamb4, lamc1, and lamc3, gradually increased from the phase III ovary stage and peaked in the early stage of gestation, especially lama4 and lama5 which showed dramatically increased expression at the blastula stage. Accordingly, in situ hybridization of lama4 was conducted. The results revealed that signals became stronger following the phase IV ovary stage, and the strongest signals were located on the follicular pseudoplacenta at the blastula stage. These results suggest that the high expression of laminin genes, especially lama4 after fertilization, may drive cell proliferation, migration, and tissue expansion in the S. schlegelii ovary and ultimately promote follicular pseudoplacenta formation.
关键词	Sebastes schlegelii follicular pseudoplacenta laminin genes evolution
DOI	10.3390/ijms231810523
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFD0901205]; National Key Research and Development Program of China[2018YFD0901204]; Research and Development Program of Shandong Province[2021LZGC029]; Shandong Province Natural Science Foundation[ZR2020KC038]; China Agriculture Research System[CARS-47]; National Marine Genetic Resource Center
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000857500700001
出版者	MDPI
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180778
专题	实验海洋生物学重点实验室
通讯作者	Liu, Qinghua; Li, Jun
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Ning,Wang, Xueying,Wang, Tao,et al. Genome-Wide Identification of Laminin Family Related to Follicular Pseudoplacenta Development in Black Rockfish (Sebastes schlegelii)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(18):12.
APA	Zhao, Ning,Wang, Xueying,Wang, Tao,Xu, Xiaojie,Liu, Qinghua,&Li, Jun.(2022).Genome-Wide Identification of Laminin Family Related to Follicular Pseudoplacenta Development in Black Rockfish (Sebastes schlegelii).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(18),12.
MLA	Zhao, Ning,et al."Genome-Wide Identification of Laminin Family Related to Follicular Pseudoplacenta Development in Black Rockfish (Sebastes schlegelii)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.18(2022):12.

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