Temperature induces metabolic reprogramming in fish during bacterial infection

doi:10.3389/fimmu.2022.1010948

IOCAS-IR > 实验海洋生物学重点实验室

	Temperature induces metabolic reprogramming in fish during bacterial infection
	Sun, Bin1,2 ; Sun, Boguang1,3 ; Zhang, Beibei1,4 ; Sun, Li 1,3,5
	2022-09-15
发表期刊	FRONTIERS IN IMMUNOLOGY
ISSN	1664-3224
卷号	13 页码:16
通讯作者	Sun, Boguang() ; Sun, Li([email protected])
摘要	Water temperature elevation as a consequence of global warming results in increased incidence of bacterial disease, such as edwardsiellosis, in fish farming. Edwardsiellosis is caused by the bacterial pathogen Edwardsiella tarda and affects many farmed fish including flounder (Paralichthys olivaceus). Currently, the effect of temperature on the metabolic response of flounder to E. tarda infection is unclear. In this study, we found that compared to low temperature (15 degrees C), high temperature (23 degrees C) enhanced E. tarda dissemination in flounder tissues. To examine the impact of temperature on the metabolism of flounder induced by E. tarda, comparative metabolomics were performed, which identified a large number of metabolites responsive to E. tarda invasion and temperature alteration. During E. tarda infection, the metabolic profile induced by elevated temperature was mainly featured by extensively decreased amino acids and TCA intermediates such as succinate, a proven immune regulator. Further, 38 potential metabolite markers of temperature effect (MMTE) in association with bacterial infection were identified. When used as exogenous supplements, two of the MMTE, i.e., L-methionine and UDP-glucose, effectively upregulated the expression of pro-inflammatory cytokines and suppressed E. tarda infection in flounder leukocytes. Taken together, the results of this study indicate an important influence of temperature on the metabolism of flounder during bacterial infection, which eventually affects the survivability of the fish.
关键词	Paralichthys olivaceus metabolism edwardsiellosis temperature global warming biomarker Edwardsiella bacterial infection
DOI	10.3389/fimmu.2022.1010948
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China; National Natural Science Foundation of China; Taishan Scholar Program of Shandong Province; [2018YFD0900500]; [41876170]
WOS研究方向	Immunology
WOS类目	Immunology
WOS记录号	WOS:000862097800001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180712
专题	实验海洋生物学重点实验室
通讯作者	Sun, Boguang; Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Fujian Polytech Normal Univ, Inst Ocean Res, Fuqing, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Qingdao Agr Univ, Coll Anim Sci & Technol, Qingdao, Peoples R China 5.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, Bin,Sun, Boguang,Zhang, Beibei,et al. Temperature induces metabolic reprogramming in fish during bacterial infection[J]. FRONTIERS IN IMMUNOLOGY,2022,13:16.
APA	Sun, Bin,Sun, Boguang,Zhang, Beibei,&Sun, Li.(2022).Temperature induces metabolic reprogramming in fish during bacterial infection.FRONTIERS IN IMMUNOLOGY,13,16.
MLA	Sun, Bin,et al."Temperature induces metabolic reprogramming in fish during bacterial infection".FRONTIERS IN IMMUNOLOGY 13(2022):16.