A pattern recognition receptor ficolin from Portunus trituberculatus (Ptficolin) regulating immune defense and hemolymph coagulation

doi:10.1016/j.ijbiomac.2022.09.041

IOCAS-IR > 实验海洋生物学重点实验室

	A pattern recognition receptor ficolin from Portunus trituberculatus (Ptficolin) regulating immune defense and hemolymph coagulation
	Liu, Yuan 1,2,3,4,6,7; Zhang, Ao 1,2,5; Guo, Na 1,2,5; Hao, Qiang 1,2,5; Li, Fuhua 1,2,3,4,6
	2022-11-30
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	221 页码:558-572
通讯作者	Liu, Yuan([email protected])
摘要	Ficolins, belonging to the fibrinogen-related protein superfamily, are important pattern recognition receptors in innate immunity. Here, a ficolin gene Ptficolin was characterized from the swimming crab Portunus trituberculatus. The completed cDNA sequence of Ptficolin encoded a signal peptide, a coiled-coil region and a fibrinogen -like domain but without the typical collagen region of vertebrate ficolins. Ptficolin showed higher expression in stomach and hepatopancreas, and presented a time-dependent response after pathogen challenge and injury stimulation. The recombinant Ptficolin (rPtficolin) could bind to various PAMPs and microorganisms, and agglutinate microorganisms and rabbit erythrocytes in a Ca2+-dependent manner, with strong binding ability to N-acetyl sugars. Meanwhile, rPtficolin promoted the hemocyte phagocytosis and clearance activity of Vibrio, while Ptficolin knockdown impaired the bacterial phagocytosis and clearance ability, suggesting the opsonin activity of Ptficolin. Knockdown of Ptficolin could downregulate the transcription of most complement-like genes and AMPs, but enhance the expression of most proPO system-related genes and key genes of Toll, IMD and JNK pathways. Moreover, knockdown of Ptficolin led to the increased hemolymph clotting time and the decreased expression of clotting-related genes. Our results indicate that Ptficolin could recognize and eliminate invading pathogens, and might be a prominent component in hemolymph coagulation of crab.
关键词	Ficolin Portunus trituberculatus Pattern recognition receptor Innate immunity Hemolymph clotting
DOI	10.1016/j.ijbiomac.2022.09.041
收录类别	SCI
语种	英语
资助项目	National Key R & D Program of China[2018YFD0901304]; National Natural Science Foundation of China[41776159]; China Agriculture Research System - 48[CARS -48]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:000859383200003
出版者	ELSEVIER
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180682
专题	实验海洋生物学重点实验室
通讯作者	Liu, Yuan
作者单位	1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Yuan,Zhang, Ao,Guo, Na,et al. A pattern recognition receptor ficolin from Portunus trituberculatus (Ptficolin) regulating immune defense and hemolymph coagulation[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,221:558-572.
APA	Liu, Yuan,Zhang, Ao,Guo, Na,Hao, Qiang,&Li, Fuhua.(2022).A pattern recognition receptor ficolin from Portunus trituberculatus (Ptficolin) regulating immune defense and hemolymph coagulation.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,221,558-572.
MLA	Liu, Yuan,et al."A pattern recognition receptor ficolin from Portunus trituberculatus (Ptficolin) regulating immune defense and hemolymph coagulation".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 221(2022):558-572.

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