Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2

doi:10.1016/j.carbpol.2022.120173

IOCAS-IR > 实验海洋生物学重点实验室

	Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2
	Geng, Lihua 1,2; Zhang, Quanbin 1,2; Suo, Qishan1,2,3 ; Wang, Jing1,2 ; Wang, Yingxia 4; Wang, Cong 5; Wu, Ning 1,2,6
	2023
发表期刊	CARBOHYDRATE POLYMERS
ISSN	0144-8617
卷号	299 页码:9
通讯作者	Wu, Ning([email protected])
摘要	COVID-19 caused by SARS-CoV-2 has spread around the world at an unprecedented rate. A more homogeneous oligo-porphyran with mean molecular weight of 2.1 kD, named OP145, was separated from Pyropia yezoensis. NMR analysis showed OP145 was mainly composed of -> 3)-beta-D-Gal-(1 -> 4)-alpha-L-Gal (6S) repeating units with few replacement of 3,6-anhydride, and the molar ratio was 1:0.85:0.11. MALDI-TOF MS revealed OP145 contained mainly tetrasulfate-oligogalactan with Dp range from 4 to 10 and with no more than two 3,6-anhydro-alpha-L-Gal replacement. The inhibitory activity of OP145 against SARS-CoV-2 was investigated in vitro and in silico. OP145 could bind to Spike glycoprotein (S-protein) through SPR result, and pseudovirus tests confirmed that OP145 could inhibite the infection with an EC50 of 37.52 mu g/mL. Molecular docking simulated the interaction between the main component of OP145 and S-protein. All the results indicated that OP145 had the potency to treat and prevent COVID-19.
关键词	Pyropia yezoensis Oligo-porphyran Oligosaccharide SARS-CoV-2 SPR Pseudovirus test
DOI	10.1016/j.carbpol.2022.120173
收录类别	SCI
语种	英语
资助项目	National Key Research and Development program of China[2019YFD0900705]; Shandong Provincial Natural Science Foundation[ZR2019BD053]; Science and Technology program of Nantong[MS12021037]; Special Open Fund of Laboratory for Marine Biology and Biotechnology; Qingdao Pilot National Laboratory for Marine Science and Technology[OF2020NO02]
WOS研究方向	Chemistry ; Polymer Science
WOS类目	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号	WOS:000866652600005
出版者	ELSEVIER SCI LTD
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180648
专题	实验海洋生物学重点实验室
通讯作者	Wu, Ning
作者单位	1.Inst Oceanol, Chinese Acad Sci, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Publ Technol Serv Ctr, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Ocean Univ China, Sch Med & Pharm, Qingdao, Peoples R China 6.Nantong Zhongke Marine Sci & Technol Res & Dev Ctr, Nantong, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Geng, Lihua,Zhang, Quanbin,Suo, Qishan,et al. Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2[J]. CARBOHYDRATE POLYMERS,2023,299:9.
APA	Geng, Lihua.,Zhang, Quanbin.,Suo, Qishan.,Wang, Jing.,Wang, Yingxia.,...&Wu, Ning.(2023).Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2.CARBOHYDRATE POLYMERS,299,9.
MLA	Geng, Lihua,et al."Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2".CARBOHYDRATE POLYMERS 299(2023):9.

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