Discovery of a novel jellyfish venom metalloproteinase inhibitor from secondary metabolites isolated from jellyfish-derived fungus Aspergillus versicolor SmT07

doi:10.1016/j.cbi.2022.110113

IOCAS-IR > 实验海洋生物学重点实验室

	Discovery of a novel jellyfish venom metalloproteinase inhibitor from secondary metabolites isolated from jellyfish-derived fungus Aspergillus versicolor SmT07
	Yue, Yang1,3 ; Yu, Huahua1,2 ; Suo, Qishan1,3,4 ; Li, Rongfeng 1,2; Liu, Song 1,2; Xing, Ronge1,2 ; Zhang, Quanbin 1,3; Li, Pengcheng1,2
	2022-09-25
发表期刊	CHEMICO-BIOLOGICAL INTERACTIONS
ISSN	0009-2797
卷号	365 页码:8
通讯作者	Yu, Huahua([email protected])
摘要	The major jellyfish stings that occur in China are caused by scyphozoan Nemopilema nomurai, whose venom exhibits significant metalloproteinase activity that contributes to the toxic effects of jellyfish envenomation. Researching effective inhibitors suppressing the metalloproteinase activity of jellyfish venom represents a new attempt to cure jellyfish envenomations. In the present study, secondary metabolites produced by the jellyfishassociated fungus Aspergillus versicolor SmT07 were isolated and evaluated for their anti-proteolytic activities. Two xanthones, sterigmatocystin (JC-01) and oxisterigmatocystin C (JC-06), and four alkaloids, cottoquinazoline A (JC-02), phenazine-1-carboxylic acid (JC-03), viridicatin (JC-04) and viridicatol (JC-05), were isolated and identified. Only phenazine-1-carboxylic acid (PCA) showed significant anti-proteolytic activity of jellyfish venom assayed on azocasein, and the IC50 value was 2.16 mM. PCA also significantly inhibited fibrinogenolytic activity, protecting the B beta chain of fibrinogen from degradation when preincubated with jellyfish venom at a ratio of >1:0.6 (PCA:venom, w/w). Molecular docking with several well-characterized snake venom metalloproteinases suggested the venom metalloproteinases inhibitory property of PCA by forming complex interactions with the active site via hydrogen bonds, 7C-7C stacking and salt bridges, which was distinct from the binding mode of batimastat. The present study represents the first study identifying natural jellyfish venom metalloproteinase inhibitors from marine natural products, which may provide an alternative to develop therapeutic agents for treating jellyfish envenomations.
关键词	Nemopilema nomurai Jellyfish envenomations Aspergillus versicolor Metalloproteinase inhibitors Molecular docking
DOI	10.1016/j.cbi.2022.110113
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41906082]; Natural Science Foundation of Shandong Province[ZR2019BD025]; Natural Science Foundation of Shandong Province[ZR2019QD012]; National Key R & D Program of China[2017YFE0111100-04]; National Key R & D Program of China[2019YFC0312605]; Major Projects on the Transformation of Old and Novel Kinetic Energy of Shandong Province[2020-1220]
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Toxicology
WOS类目	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Toxicology
WOS记录号	WOS:000874830700005
出版者	ELSEVIER IRELAND LTD
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180558
专题	实验海洋生物学重点实验室
通讯作者	Yu, Huahua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Pilot Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yue, Yang,Yu, Huahua,Suo, Qishan,et al. Discovery of a novel jellyfish venom metalloproteinase inhibitor from secondary metabolites isolated from jellyfish-derived fungus Aspergillus versicolor SmT07[J]. CHEMICO-BIOLOGICAL INTERACTIONS,2022,365:8.
APA	Yue, Yang.,Yu, Huahua.,Suo, Qishan.,Li, Rongfeng.,Liu, Song.,...&Li, Pengcheng.(2022).Discovery of a novel jellyfish venom metalloproteinase inhibitor from secondary metabolites isolated from jellyfish-derived fungus Aspergillus versicolor SmT07.CHEMICO-BIOLOGICAL INTERACTIONS,365,8.
MLA	Yue, Yang,et al."Discovery of a novel jellyfish venom metalloproteinase inhibitor from secondary metabolites isolated from jellyfish-derived fungus Aspergillus versicolor SmT07".CHEMICO-BIOLOGICAL INTERACTIONS 365(2022):8.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1-s2.0-S000927972200（4816KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览