Both AMP-activated and cAMP-dependent protein kinase regulate the expression of heat shock protein 70-2 gene in Neopyropia yezoensis

doi:10.3389/fmars.2022.1004600

IOCAS-IR > 实验海洋生物学重点实验室

	Both AMP-activated and cAMP-dependent protein kinase regulate the expression of heat shock protein 70-2 gene in Neopyropia yezoensis
	Sun, Zhenjie 1,2; Gu, Wenhui 2,3,4; Feng, Zezhong 2; Fan, Yaqin 1; Niu, Jianfeng 2,3,4; Wang, Guangce 2,3,4
	2022-09-26
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	9 页码:13
通讯作者	Niu, Jianfeng([email protected]) ; Wang, Guangce([email protected])
摘要	The upregulation of heat shock protein 70 (hsp70) gene under high temperature stress is a common phenomenon. Although heat shock protein-mediated stress responses play an important role in intertidal Neopyropia yezoensis, the detailed regulatory mechanism of the hsp70 gene expression is still unclear. Here, a full-length sequence of the hsp70-2 gene was cloned and its' expression regulation was analyzed. There was an activating transcription factors element (ATFE) of cAMP-dependent protein kinase (PKA) was found at the gene promoter region and a highly conserved deduced amino acid sequence with calmodulin-binding activity was detected. Reagents implicated in the induction of the hsp70 gene were then selected to treat the algal samples at 24 degrees C, and the photosynthetic parameters, transcription and translation of this gene were determined. Results showed that quercetin inhibited the transcription of the hsp70-2 gene, significantly decreased the synthesis of the HSP70-2 protein, and lowered the photosynthetic activity of N. yezoensis under high temperature stress conditions. Although the addition of trifluoperazine (TFP), an inhibitor of calmodulin (CAM), downregulated the photosynthetic parameters, the transcription of the hsp70-2 gene was not influenced at high temperature treatment, implying that CAM was not involved in the transcription of the hsp70-2 gene but involved in the heat stress reponding pathways. 5 '-aminoimidasole-4-carboxamide-1-beta-d-ribofuranoside (AICAR) promoted the synthesis of hsp70-2 transcripts significantly and HSP70-2 protein slightly, which indicated that AMPK regulated the induction of the hsp70-2 gene in N. yezoensis. Forskolin also promoted the upregulation of the hsp70-2 gene. Thus, both AMPK and cAMP-dependent protein kinase (PKA) could phosphorylate HSF and activate the expression of the hsp70-2 gene in N. yezoensis. However, there was no strict correlation between transcripts of the hsp70-2 gene and HSP70-2 protein contents. It was proposed that the posttranscriptional mechanisms were involved in regulating the synthesis of the HSP70-2 protein. Which allowed it to adapt to the extremely variable intertidal environments with an instant responding manner, since the accumulated transcripts could be translated rapidly when needed.
关键词	heat shock protein 70 high-temperature stress AMP-activated protein kinase cAMP-dependent protein kinase Neopyropia yezoensis
DOI	10.3389/fmars.2022.1004600
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901500]; China Agriculture Research System of MOF; Key Deployment Project of the Centre for Ocean Mega-Research of Science; Chinese Academy of Sciences[COMS2019Q02]; Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2019JZZY010815]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000874200900001
出版者	FRONTIERS MEDIA SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180555
专题	实验海洋生物学重点实验室
通讯作者	Niu, Jianfeng; Wang, Guangce
作者单位	1.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China 2.Chinese Acad Sci IOCAS, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Lab Marine Biol & Biotechnol, Pilot Natl Lab Marine Sci & Technol, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, Zhenjie,Gu, Wenhui,Feng, Zezhong,et al. Both AMP-activated and cAMP-dependent protein kinase regulate the expression of heat shock protein 70-2 gene in Neopyropia yezoensis[J]. FRONTIERS IN MARINE SCIENCE,2022,9:13.
APA	Sun, Zhenjie,Gu, Wenhui,Feng, Zezhong,Fan, Yaqin,Niu, Jianfeng,&Wang, Guangce.(2022).Both AMP-activated and cAMP-dependent protein kinase regulate the expression of heat shock protein 70-2 gene in Neopyropia yezoensis.FRONTIERS IN MARINE SCIENCE,9,13.
MLA	Sun, Zhenjie,et al."Both AMP-activated and cAMP-dependent protein kinase regulate the expression of heat shock protein 70-2 gene in Neopyropia yezoensis".FRONTIERS IN MARINE SCIENCE 9(2022):13.

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