A Sensitive and Portable Double-Layer Microfluidic Biochip for Harmful Algae Detection

doi:10.3390/mi13101759

	A Sensitive and Portable Double-Layer Microfluidic Biochip for Harmful Algae Detection
	Li, Ping 1; Qiang, Le 1,2,3; Han, Yingkuan 1; Chu, Yujin 1; Qiu, Jiaoyan 1; Song, Fangteng 1; Wang, Min 1; He, Qihang 1; Zhang, Yunhong 1; Sun, Mingyuan 1; Li, Caiwen4 ; Song, Shuqun 4; Liu, Yun 4; Han, Lin 1; Zhang, Yu1
	2022-10-01
发表期刊	MICROMACHINES
卷号	13 期号:10 页码:13
通讯作者	Han, Lin([email protected]) ; Zhang, Yu([email protected])
摘要	Harmful algal blooms (HABs) are common disastrous ecological anomalies in coastal waters. An effective algae monitoring approach is important for natural disaster warning and environmental governance. However, conducting rapid and sensitive detection of multiple algae is still challenging. Here, we designed an ultrasensitive, rapid and portable double-layer microfluidic biochip for the simultaneous quantitative detection of six species of algae. Specific DNA probes based on the 18S ribosomal DNA (18S rDNA) gene fragments of HABs were designed and labeled with the fluorescent molecule cyanine-3 (Cy3). The biochip had multiple graphene oxide (GO) nanosheets-based reaction units, in which GO nanosheets were applied to transfer target DNA to the fluorescence signal through a photoluminescence detection system. The entire detection process of multiple algae was completed within 45 min with the linear range of fluorescence recovery of 0.1 fM-100 nM, and the detection limit reached 108 aM. The proposed approach has a simple detection process and high detection performance and is feasible to conduct accurate detection with matched portable detection equipment. It will have promising applications in marine natural disaster monitoring and environmental care.
关键词	microfluidic biochip harmful algal bloom nuclei acids sensing environmental warning photoluminescence detection
DOI	10.3390/mi13101759
收录类别	SCI
语种	英语
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2021QNLM040001-2]; NationalKey RD Plan of China[2019YFC1407800]; Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in the Universities of Shandong
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation ; Physics
WOS类目	Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation ; Physics, Applied
WOS记录号	WOS:000875134500001
出版者	MDPI
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180522
专题	海洋生态与环境科学重点实验室
通讯作者	Han, Lin; Zhang, Yu
作者单位	1.Shandong Univ, Inst Marine Sci & Technol, Qingdao 266000, Peoples R China 2.Xi An Jiao Tong Univ, Coll Stomatol, Key Lab Shaanxi Prov Craniofacial Precis Med Res, Xian 710004, Peoples R China 3.Xi An Jiao Tong Univ, Hosp Stomatol, Dept Endodont, Xian 710004, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li, Ping,Qiang, Le,Han, Yingkuan,et al. A Sensitive and Portable Double-Layer Microfluidic Biochip for Harmful Algae Detection[J]. MICROMACHINES,2022,13(10):13.
APA	Li, Ping.,Qiang, Le.,Han, Yingkuan.,Chu, Yujin.,Qiu, Jiaoyan.,...&Zhang, Yu.(2022).A Sensitive and Portable Double-Layer Microfluidic Biochip for Harmful Algae Detection.MICROMACHINES,13(10),13.
MLA	Li, Ping,et al."A Sensitive and Portable Double-Layer Microfluidic Biochip for Harmful Algae Detection".MICROMACHINES 13.10(2022):13.

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micromachines-13-017（5641KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览