Maternal nutrients and N-15 Leucine tracing in Sebastes schlegelii

doi:10.3389/fmars.2022.937805

IOCAS-IR > 实验海洋生物学重点实验室

	Maternal nutrients and N-15 Leucine tracing in Sebastes schlegelii
	Du, Tengfei1,2,3,4 ; Xiao, Yongshuang 1,2,3,5; Liu, Qinghua 1,2,3; Guo, Teng 1,2,3,6; Gao, Guang1,2,3,4 ; Li, Jun 1,2,3,5
	2022-10-18
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	9 页码:15
通讯作者	Xiao, Yongshuang([email protected]) ; Li, Jun([email protected])
摘要	Sebastes schlegelii is a commercially important fishery resource that is also a representative viviparous fish species in the Northeast Asian Sea. The mode of nutritional supply of embryos fertilized and developed in vivo is not clear. To screen biomarkers for nutrient transfer between mother and embryo, we studied the composition of and changes in maternal plasma at different developmental stages. The results showed that the plasma of S. schlegelii was rich in nutrients, including 16 hydrolysed amino acids, 20 fatty acids, glucose and fructose. The contents of amino acids in the plasma reached 3.44-4.58 g/100 g, which is approximately 3-5 times that of fatty acids and 44-155 times that of sugar. Thirteen amino acids, 15 fatty acids and glucose showed an increasing trend in the early stage of embryonic development and a decreasing trend in the later stage. The contents of leucine, as an essential amino acid in early development, changed significantly in maternal blood and was therefore selected as a biomarker. The results of isotope labelling showed that N-15 leucine was not detected in the eggs, gastrula embryos or organogenesis embryos, but a high abundance of N-14 leucine was detected in the eggs and embryos of the control and experimental groups, indicating that leucine, an essential amino acid for aquatic animals, plays an important role in gamete and embryo development. An abundance in N-15-labelled leucine was detected in the embryonic blood and ovarian villi but not in the embryos, which indicates that the ample free naturally abundant leucine in maternal blood during the middle and late developmental stages of the eggs and embryos was not absorbed or utilized. These results showed that eggs and embryos had certain autonomy and selectivity when absorbing nutrients and gave priority to the active transport of certain complex macromolecular nutrients that could meet their needs for a variety of nutrients at the same time.
关键词	Sebastes schlegelii maternal nutrition nutrients stable isotope plasma
DOI	10.3389/fmars.2022.937805
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program[2018YFD0901204]; Key Deployment Projects of Center for Ocean Mega-Science, Chinese Academy of Sciences[COMS2020Q05]; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[GML2019ZD0402]; China Agriculture Research System[CARS-47]; Major Agricultural Application Technology Innovation Project of Shandong Province[SD2019YY011]; STS project[KFZD-SW-106]; STS project[ZSSD-019]; STS project[2017T3017]; STS project[KFJ-STS-QYZX-020]; Qingdao National Laboratory for Marine Science and Technology[2018SDKJ0502-2]; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000878527600001
出版者	FRONTIERS MEDIA SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180429
专题	实验海洋生物学重点实验室
通讯作者	Xiao, Yongshuang; Li, Jun
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Southern Marine Sci & Engn Guangdong Lab Guangzhou, Guangzhou, Peoples R China 6.Qingdao Agr Univ, Sch Life Sci & Engn, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Du, Tengfei,Xiao, Yongshuang,Liu, Qinghua,et al. Maternal nutrients and N-15 Leucine tracing in Sebastes schlegelii[J]. FRONTIERS IN MARINE SCIENCE,2022,9:15.
APA	Du, Tengfei,Xiao, Yongshuang,Liu, Qinghua,Guo, Teng,Gao, Guang,&Li, Jun.(2022).Maternal nutrients and N-15 Leucine tracing in Sebastes schlegelii.FRONTIERS IN MARINE SCIENCE,9,15.
MLA	Du, Tengfei,et al."Maternal nutrients and N-15 Leucine tracing in Sebastes schlegelii".FRONTIERS IN MARINE SCIENCE 9(2022):15.

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