Insights into symbiotic interactions from metatranscriptome analysis of deep-sea mussel Gigantidas platifrons under long-term laboratory maintenance

doi:10.1111/mec.16765

	Insights into symbiotic interactions from metatranscriptome analysis of deep-sea mussel Gigantidas platifrons under long-term laboratory maintenance
	Sun, Yan 1,2,3,4; Wang, Minxiao 1,2,3,4,6,7; Chen, Hao1,2,3,4 ; Wang, Hao 1,2,3,4; Zhong, Zhaoshan1,2,3,4 ; Zhou, Li 1,2,3,4; Fu, Lulu 1,2,3,4; Li, Chaolun1,2,3,4,5 ; Sun, Song1,2,3,4,5,6,7
	2022-11-17
发表期刊	MOLECULAR ECOLOGY
ISSN	0962-1083
页码	16
通讯作者	Wang, Minxiao([email protected]) ; Sun, Song([email protected])
摘要	Symbioses between invertebrates and chemosynthetic bacteria are of fundamental importance in deep-sea ecosystems, but the mechanisms that enable their symbiont associations are still largely undescribed, owing to the culturable difficulties of deep-sea lives. Bathymodiolinae mussels are remarkable in their ability to overcome decompression and can be maintained successfully for an extended period under atmospheric pressure, thus providing a model for investigating the molecular basis of symbiotic interactions. Herein, we conducted metatranscriptome sequencing and gene co-expression network analysis of Gigantidas platifrons under laboratory maintenance with gradual loss of symbionts. The results revealed that one-day short-term maintenance triggered global transcriptional perturbation in symbionts, but little gene expression changes in mussel hosts, which were mainly involved in responses to environmental changes. Long-term maintenance with depleted symbionts induced a metabolic shift in the mussel host. The most notable changes were the suppression of sterol biosynthesis and the complementary activation of terpenoid backbone synthesis in response to the reduction of bacteria-derived terpenoid sources. In addition, we detected the upregulation of host proteasomes responsible for amino acid deprivation caused by symbiont depletion. Additionally, a significant correlation between host microtubule motor activity and symbiont abundance was revealed, suggesting the possible function of microtubule-based intracellular trafficking in the nutritional interaction of symbiosis. Overall, by analyzing the dynamic transcriptomic changes during the loss of symbionts, our study highlights the nutritional importance of symbionts in supplementing terpenoid compounds and essential amino acids and provides insight into the molecular mechanisms and strategies underlying the symbiotic interactions in deep-sea ecosystems.
关键词	Bathymodiolinae mussel deep-sea endosymbioses gene network analysis laboratory maintenance metatranscriptome symbiotic interaction
DOI	10.1111/mec.16765
收录类别	SCI
语种	英语
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao); National Natural Science Foundation of China; Mount Tai Scholar Climbing Plan; [2022QNLM030004]; [41806200]; [42076091]; [42030407]; [41906124]
WOS研究方向	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS类目	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS记录号	WOS:000888413200001
出版者	WILEY
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180342
专题	海洋生态与环境科学重点实验室
通讯作者	Wang, Minxiao; Sun, Song
作者单位	1.Chinese Acad Sci, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Chinese Acad Sci, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 7.Inst Oceanol, Chinese Acad Sci, Ctr Deep Sea Res, Qingdao 266071, Peoples R China
第一作者单位	深海极端环境与生命过程研究中心
通讯作者单位	深海极端环境与生命过程研究中心; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, Yan,Wang, Minxiao,Chen, Hao,et al. Insights into symbiotic interactions from metatranscriptome analysis of deep-sea mussel Gigantidas platifrons under long-term laboratory maintenance[J]. MOLECULAR ECOLOGY,2022:16.
APA	Sun, Yan.,Wang, Minxiao.,Chen, Hao.,Wang, Hao.,Zhong, Zhaoshan.,...&Sun, Song.(2022).Insights into symbiotic interactions from metatranscriptome analysis of deep-sea mussel Gigantidas platifrons under long-term laboratory maintenance.MOLECULAR ECOLOGY,16.
MLA	Sun, Yan,et al."Insights into symbiotic interactions from metatranscriptome analysis of deep-sea mussel Gigantidas platifrons under long-term laboratory maintenance".MOLECULAR ECOLOGY (2022):16.

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