The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus)

doi:10.3390/microorganisms10112175

IOCAS-IR > 实验海洋生物学重点实验室

	The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus)
	Shi, Xueyan 1; Chi, Heng 1,2; Sun, Yuanyuan3 ; Tang, Xiaoqian 1,2; Xing, Jing 1,2; Sheng, Xiuzhen 1,2; Zhan, Wenbin 1,2
	2022-11-01
发表期刊	MICROORGANISMS
卷号	10 期号:11 页码:16
通讯作者	Chi, Heng([email protected])
摘要	The peritoneal cavity plays an important role in the immune response, and intraperitoneal administration is an ideal vaccination route in fish. However, immune responses in the peritoneal cavity of teleost fish are still not completely characterized. This study characterized the morphology of peritoneal cavity cells (PerC cells) and their composition in flounder (Paralichthys olivaceus). Flow cytometric analysis of the resident PerC cells revealed two populations varying in granularity and size. One population, approximately 15.43% +/- 1.8%, was smaller with a lower granularity, designated as lymphocytes. The other population of the cells, about 78.17% +/- 3.52%, was larger with higher granularity and was designated as myeloid cells. The results of cytochemical staining and transmission electron microscopy indicated that peritoneal cavity in flounder normally contains a resident population of leukocytes dominated by granulocytes, macrophages, dendritic cells, and lymphocytes. The percentages of IgM(+), CD4(+), G-CSFR+, MHCII+, and CD83(+) leukocytes among PerC cells determined by flow cytometry were 3.13% +/- 0.4%, 2.83% +/- 0.53%, 21.12% +/- 1.44%, 27.11% +/- 3.30%, and 19.64% +/- 0.31%, respectively. Further, the changes in IgM(+), CD4(+), G-CSFR+, MHCII+, and CD83(+) leukocytes in flounder after Vibrio anguillarum infection and immunization were compared. The composition changed rapidly after the infection or vaccination treatment and included two stages, a non-specific stage dominated by phagocytes and a specific immune stage dominated by lymphocytes. Due to the virulence effectors of bacteria, the infected group exhibited a more intense and complicated PerC cells immune response than that of the immunization group. Following our previous study, this is the first report on the morphology and composition of PerC cells and the early activation of PerC cells in flounder response to V. anguillarum infection and vaccination.
关键词	peritoneal cavity cells cell composition infection vaccination fish
DOI	10.3390/microorganisms10112175
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2019YFD0900101]; National Natural Science Foundation of China[31872594]; National Natural Science Foundation of China[41906108]; National Natural Science Foundation of China[31730101]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000885818500001
出版者	MDPI
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180257
专题	实验海洋生物学重点实验室
通讯作者	Chi, Heng
作者单位	1.Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, 5 Yushan Rd, Qingdao 266003, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Shi, Xueyan,Chi, Heng,Sun, Yuanyuan,et al. The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus)[J]. MICROORGANISMS,2022,10(11):16.
APA	Shi, Xueyan.,Chi, Heng.,Sun, Yuanyuan.,Tang, Xiaoqian.,Xing, Jing.,...&Zhan, Wenbin.(2022).The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus).MICROORGANISMS,10(11),16.
MLA	Shi, Xueyan,et al."The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus)".MICROORGANISMS 10.11(2022):16.

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