IDENTIFICATION OF A GENOMIC REGION LINKED WITH SEX DETERMINATION OF UNDARIA PINNATIFIDA (ALARIACEAE) THROUGH GENOMIC RESEQUENCING AND GENETIC LINKAGE ANALYSES OF A SEGREGATING GAMETOPHYTE FAMILY

doi:10.1111/jpy.13295

IOCAS-IR > 实验海洋生物学重点实验室

	IDENTIFICATION OF A GENOMIC REGION LINKED WITH SEX DETERMINATION OF UNDARIA PINNATIFIDA (ALARIACEAE) THROUGH GENOMIC RESEQUENCING AND GENETIC LINKAGE ANALYSES OF A SEGREGATING GAMETOPHYTE FAMILY
	Shan, Tifeng 1,2; Li, Yuqian1,3 ; Pang, Shaojun 1,2
	2022-11-29
发表期刊	JOURNAL OF PHYCOLOGY
ISSN	0022-3646
页码	11
通讯作者	Pang, Shaojun([email protected])
摘要	Different from the traditional knowledge about kelp, three sexual phenotypes (female, male, and monoecious) exist in the haploid gametophytes of Undaria pinnatifida. However, the sex-determining mechanisms remain unknown. Genetic linkage mapping is an efficient tool to identify sex-linked regions. In the present study, we resequenced a segregating gametophyte family based on the male genome of U. pinnatifida. A high-density genetic linkage map was constructed using 9887 SNPs, with an average distance of 0.41 cM between adjacent SNPs. On the basis of this genetic map and using the composite interval mapping method, we identified 62 SNPs significantly linked with the sexual phenotype. They were located at a position of 67.67 cM on the linkage group 23, corresponding to a physical range of 14.67 Mbp on the HiC_Scaffold_23 of the genome. Reanalysis of the previous specific length amplified fragment sequencing data according to the reference genome led to the identification of a sex-linked genomic region that encompassed the above-mentioned 14.67 Mbp region. Hence, this overlapped genomic range was likely the sex-determining region. Within this region, 129 genes were retrieved and 39 of them were annotated with explicit function, including the potential male sex-determining gene-encoding high mobility group (HMG) domain protein. Relative expression analysis of the HMG gene showed that its expression was higher in male gametophytes during the vegetative phase and monoecious gametophytes during both the vegetative and gametogenesis phases, but significantly lower in male gametophytes during the gametogenesis phase. These results provide a foundation for deciphering the sex-determining mechanism of U. pinnatifida.
关键词	brown alga genetic linkage map kelp monoecious sex determination SNP
DOI	10.1111/jpy.13295
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42176100]; Chinese Academy of Sciences[KFJ-BRP-017-27]; China Agriculture Research System[CARS-50]; Taishan Scholar Program of Shandong Province; Foundation for Huiquan Scholar of Institute of Oceanology, Chinese Academy of Sciences
WOS研究方向	Plant Sciences ; Marine & Freshwater Biology
WOS类目	Plant Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000892266300001
出版者	WILEY
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180238
专题	实验海洋生物学重点实验室
通讯作者	Pang, Shaojun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Shan, Tifeng,Li, Yuqian,Pang, Shaojun. IDENTIFICATION OF A GENOMIC REGION LINKED WITH SEX DETERMINATION OF UNDARIA PINNATIFIDA (ALARIACEAE) THROUGH GENOMIC RESEQUENCING AND GENETIC LINKAGE ANALYSES OF A SEGREGATING GAMETOPHYTE FAMILY[J]. JOURNAL OF PHYCOLOGY,2022:11.
APA	Shan, Tifeng,Li, Yuqian,&Pang, Shaojun.(2022).IDENTIFICATION OF A GENOMIC REGION LINKED WITH SEX DETERMINATION OF UNDARIA PINNATIFIDA (ALARIACEAE) THROUGH GENOMIC RESEQUENCING AND GENETIC LINKAGE ANALYSES OF A SEGREGATING GAMETOPHYTE FAMILY.JOURNAL OF PHYCOLOGY,11.
MLA	Shan, Tifeng,et al."IDENTIFICATION OF A GENOMIC REGION LINKED WITH SEX DETERMINATION OF UNDARIA PINNATIFIDA (ALARIACEAE) THROUGH GENOMIC RESEQUENCING AND GENETIC LINKAGE ANALYSES OF A SEGREGATING GAMETOPHYTE FAMILY".JOURNAL OF PHYCOLOGY (2022):11.

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