Reviving and characterizing three species of dinoflagellate cysts dormant for about 70 years in the East China Sea: Biecheleria brevisulcata, Biecheleriopsis adriatica, and Scrippsiella donghaienis

doi:10.1007/s00343-022-2122-1

	Reviving and characterizing three species of dinoflagellate cysts dormant for about 70 years in the East China Sea: Biecheleria brevisulcata, Biecheleriopsis adriatica, and Scrippsiella donghaienis
	Hu, Zhangxi1,2,3,6 ; Song, Xiaoying2,7 ; Wang, Jinxiu2,7 ; Tao, Zhe 2,7; Sun, Yuanyuan4 ; Li, Yuhang5 ; Liu, Yuyang 2; Deng, Yunyan 2,3,6; Shang, Lixia 2,3,6; Chai, Zhaoyang 2,3,6; Tang, Yingzhong 2,3,6
	2022-11-24
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
页码	20
通讯作者	Tang, Yingzhong([email protected])
摘要	Many marine dinoflagellates can form resting cysts as a part of their life cycle, and the cysts could be buried in sediment and remained viable for as long as over 150 years. However, only a very limited number of cyst species have been revived from long-buried sediments and investigated in regard to a possible shift in the intra-specific genetic structure of a species detected from the historical record at a particular location. Here, we report a successful germination of three species of resting cysts that were sampled from the depth dated back to 1941 +/- 18 AD from a 44-cm sediment core from the East China Sea. Seven isolates were established from germination of single cyst isolation or multi-cyst germinations. LSU rRNA gene or ITS sequences of these strains were obtained, then they were identified to be Biecheleria brevisulcata (five strains), Biecheleriopsis adriatica (one strain), and Scrippsiella donghaienis (one strain) in terms of morphology and rRNA gene sequence. Biecheleria brevisulcata strain 1, Bps. adriatica strain 21, and S. donghaienis strain 23 were examined in detail with light microscope (LM) and scanning electron microscope (SEM), and analyzed with high performance liquid chromatography (HPLC) for their pigment compositions, and genetic diversity. We also confirmed the presence of a resting cyst of Bps. adriatica in the field for the first time. The LSU rRNA gene-based genetic distances of Bps. adriatica from that obtained from water sample, single-cell PCR sequencing for the cysts isolated from the surface sediment of the same sea area and that reported from other regions during the recent years, and ITS-based genetic distances of S. donghaienis from that obtained from cysts isolated from the surface sediment of the same location and that reported from other regions during the recent years indicated that the intra-specific genetic structure of each species in the sampling area may have shifted during the last 70 years. Our work confirms that B. brevisulcata, Bps. adriatica, and S. donghaienis, all described as new species around 2010, have inhabited the East China Sea for about 70 years. The present work reports for the first time the revival of dinoflagellate resting cysts long-buried in the coastal sediments of China, which facilitates further study on the historical occurrences of other harmful dinoflagellates and their relevance to the regional climate and environmental changes in China.
关键词	core sediment dinoflagellate resting cyst germination Biecheleria brevisulcata Biecheleriopsis adriatica Scrippsiella donghaienis
DOI	10.1007/s00343-022-2122-1
收录类别	SCI
语种	英语
资助项目	Science and Technology Basic Resources Investigation Program of China[2018FY100200]; National Natural Science Foundation of China[41976134]; Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao)[LMEES-YTSP-2018-01-04]; Program for Scientific Research Start-up Funds of Guangdong Ocean University[060302022201]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000888684200003
出版者	SCIENCE PRESS
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180229
专题	海洋生态与环境科学重点实验室实验海洋生物学重点实验室海洋生物分类与系统演化实验室
通讯作者	Tang, Yingzhong
作者单位	1.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China 6.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 7.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Hu, Zhangxi,Song, Xiaoying,Wang, Jinxiu,et al. Reviving and characterizing three species of dinoflagellate cysts dormant for about 70 years in the East China Sea: Biecheleria brevisulcata, Biecheleriopsis adriatica, and Scrippsiella donghaienis[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2022:20.
APA	Hu, Zhangxi.,Song, Xiaoying.,Wang, Jinxiu.,Tao, Zhe.,Sun, Yuanyuan.,...&Tang, Yingzhong.(2022).Reviving and characterizing three species of dinoflagellate cysts dormant for about 70 years in the East China Sea: Biecheleria brevisulcata, Biecheleriopsis adriatica, and Scrippsiella donghaienis.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,20.
MLA	Hu, Zhangxi,et al."Reviving and characterizing three species of dinoflagellate cysts dormant for about 70 years in the East China Sea: Biecheleria brevisulcata, Biecheleriopsis adriatica, and Scrippsiella donghaienis".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2022):20.

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