New Evidence for the Existence of Two Kiss/Kissr Systems in a Flatfish Species, the Turbot (Scophthalmus maximus), and Stimulatory Effects on Gonadotropin Gene Expression

doi:10.3389/fendo.2022.883608

IOCAS-IR > 实验海洋生物学重点实验室

	New Evidence for the Existence of Two Kiss/Kissr Systems in a Flatfish Species, the Turbot (Scophthalmus maximus), and Stimulatory Effects on Gonadotropin Gene Expression
	Zhao, Chunyan1,2,3 ; Wang, Bin4 ; Liu, Yifan 2,3,5; Feng, Chengcheng2,3 ; Xu, Shihong 2,3; Wang, Wenqi 1; Liu, Qinghua 2,3; Li, Jun 2,3,6
	2022-06-15
发表期刊	FRONTIERS IN ENDOCRINOLOGY
ISSN	1664-2392
卷号	13 页码:17
通讯作者	Li, Jun([email protected])
摘要	Seasonal reproduction is generally controlled by the hypothalamus-pituitary-gonadal (HPG) axis in fish. Previous studies have demonstrated that the kisspeptin (Kiss)/kisspeptin receptor (Kissr) system, a positive regulator of the HPG axis, mediates the responses to environmental cues. Turbot (Scophthalmus maximus), a representative species of Pleuronectiformes, is one of the most commercially important fish species cultured in Europe and North China. However, the mechanisms by which the Kiss/Kissr system regulates the reproductive axis of turbot according to seasonal changes, especially photoperiod, have not been clearly characterized. In the current study, the cDNA sequences of kiss2/kissr2, along with kiss1/kissr3 which was thought to be lost in flatfish species, were cloned and functionally characterized. The kiss1, kiss2, and kissr3 transcripts were highly detected in the brain and gonad, while kissr2 mRNA was only abundantly expressed in the brain. Moreover, kiss/kissr mRNAs were further examined in various brain areas of both sexes. The kiss1, kissr2, kissr3 mRNAs were highly expressed in the mesencephalon, while a substantial degree of kiss2 transcripts were observed in the hypothalamus. During annual reproductive cycle, both kiss and kissr transcript levels declined significantly from the immature to mature stages and increased at the degeneration stage in the brains of both sexes, especially in the mesencephalon and hypothalamus. The ovarian kiss1, kiss2, and kissr2 mRNA levels were highest at the vitellogenic stage (mature stage), while expression of kissr3 was highest at the immature stage. The testicular kiss and kissr transcripts were highest in the immature and degeneration stages, and lowest at the mature stage. In addition, intraperitoneal injection of Kiss1-10 and Kiss2-10 significantly stimulated mRNA levels of pituitary lh beta, fhs beta, and gth alpha. In summary, two Kiss/Kissr systems were firstly proven in a flatfish species of turbot, and it has a positive involvement in controlling the reproduction of the Kiss/Kissr system in turbot. The results will provide preliminary information regarding how the Kiss/Kissr system controls seasonal reproduction in turbot broodstock.
关键词	turbot kisspeptin kisspeptin receptor reproduction gonadal development
DOI	10.3389/fendo.2022.883608
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31802319]; National Natural Science Foundation of China[32072949]; National Natural Science Foundation of China[31702321]; Advanced Talents Foundation of QAU[6631119055]; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[GML2019ZD0402]; China Agriculture Research System[CARS-47]
WOS研究方向	Endocrinology & Metabolism
WOS类目	Endocrinology & Metabolism
WOS记录号	WOS:000817985300001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179632
专题	实验海洋生物学重点实验室
通讯作者	Li, Jun
作者单位	1.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr & Rural Affairs, Qingdao, Peoples R China 5.Zhejiang Ocean Univ, Natl Engn Res Ctr Marine Aquaculture, Zhoushan, Peoples R China 6.Southern Marine Sci & Engn Guangdong Lab Guangzhou, Guangzhou, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhao, Chunyan,Wang, Bin,Liu, Yifan,et al. New Evidence for the Existence of Two Kiss/Kissr Systems in a Flatfish Species, the Turbot (Scophthalmus maximus), and Stimulatory Effects on Gonadotropin Gene Expression[J]. FRONTIERS IN ENDOCRINOLOGY,2022,13:17.
APA	Zhao, Chunyan.,Wang, Bin.,Liu, Yifan.,Feng, Chengcheng.,Xu, Shihong.,...&Li, Jun.(2022).New Evidence for the Existence of Two Kiss/Kissr Systems in a Flatfish Species, the Turbot (Scophthalmus maximus), and Stimulatory Effects on Gonadotropin Gene Expression.FRONTIERS IN ENDOCRINOLOGY,13,17.
MLA	Zhao, Chunyan,et al."New Evidence for the Existence of Two Kiss/Kissr Systems in a Flatfish Species, the Turbot (Scophthalmus maximus), and Stimulatory Effects on Gonadotropin Gene Expression".FRONTIERS IN ENDOCRINOLOGY 13(2022):17.