Regulation of the Cell Cycle, Apoptosis, and Proline Accumulation Plays an Important Role in the Stress Response of the Eastern Oyster Crassostrea Virginica

doi:10.3389/fmars.2022.921877

	Regulation of the Cell Cycle, Apoptosis, and Proline Accumulation Plays an Important Role in the Stress Response of the Eastern Oyster Crassostrea Virginica
	Li, Cui1,2,3 ; Wang, Haiyan 2,3; Guo, Ximing 1
	2022-06-17
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	9 页码:12
通讯作者	Guo, Ximing([email protected])
摘要	BackgroundUnderstanding how organisms respond and adapt to environmental changes is central to evolutionary biology. As a sessile organism that has adapted to life in estuaries and intertidal zones, the eastern oyster Crassostrea virginica can tolerate wide fluctuations in temperature and salinity and survive for weeks out of water. To understand the molecular mechanisms underlying the remarkable stress tolerance of the eastern oyster, we studied the transcriptomic changes induced by exposure to air and cold stress. Eastern oysters were maintained for 7 days under four conditions, namely, in seawater (normal) at 22 degrees C, in air at 22 degrees C, in seawater at 5 degrees C and in air at 5 degrees C, and then sampled for RNA sequencing. ResultsTranscriptomic analysis revealed that many genes involved in cell cycle progression and DNA replication were downregulated in oysters exposed to air and cold, which indicates that stress inhibits cell division. Exposure to air at 22 degrees C induced a concerted inhibition of apoptosis through the upregulation of expanded inhibitors of apoptosis and the downregulation of caspases. Interactions between TNF and NF-kappa B signalling implied a reduction in the inflammatory response and immune functions. Key genes for proline production, fatty acid synthesis and chromosomal proteins were upregulated during exposure to low temperatures, which suggested that proline accumulation, energy conservation, and epigenetic modification of chromosomes are important for coping with cold stress. The upregulation of melatonin, FMRFamide, and neural acetylcholine receptors indicate the significance of the neurohormonal regulation of homeostasis. ConclusionThese results show that air exposure and cold stress alter the expression of key genes for cell division, apoptosis, proline accumulation, fatty acid metabolism, neurohormonal signalling, and epigenetic modifications, suggesting regulation of these processes plays an important role in the stress response of the eastern oyster and possibly other marine molluscs. This study provides new insights into molecular mechanisms of stress response that are essential for understanding the adaptive potential of marine organisms under climate change.
关键词	stress transcriptome cell cycle control apoptosis growth immune function epigenetic modification
DOI	10.3389/fmars.2022.921877
收录类别	SCI
语种	英语
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000820049900001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179620
专题	海洋生物分类与系统演化实验室
通讯作者	Guo, Ximing
作者单位	1.Rutgers State Univ, Dept Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08854 USA 2.Chinese Acad Sci, Inst Oceanol, Dept Marine Organism Taxon & Phylogeny, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Cui,Wang, Haiyan,Guo, Ximing. Regulation of the Cell Cycle, Apoptosis, and Proline Accumulation Plays an Important Role in the Stress Response of the Eastern Oyster Crassostrea Virginica[J]. FRONTIERS IN MARINE SCIENCE,2022,9:12.
APA	Li, Cui,Wang, Haiyan,&Guo, Ximing.(2022).Regulation of the Cell Cycle, Apoptosis, and Proline Accumulation Plays an Important Role in the Stress Response of the Eastern Oyster Crassostrea Virginica.FRONTIERS IN MARINE SCIENCE,9,12.
MLA	Li, Cui,et al."Regulation of the Cell Cycle, Apoptosis, and Proline Accumulation Plays an Important Role in the Stress Response of the Eastern Oyster Crassostrea Virginica".FRONTIERS IN MARINE SCIENCE 9(2022):12.