UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus

doi:10.3389/fpls.2022.862594

IOCAS-IR

	UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus
	Wang, Min 1; Du, Guicai 2; Fang, Junna 3; Wang, Linsong 4; Guo, Qunqun 2; Zhang, Tingting 2; Li, Ronggui 2
	2022-05-31
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	13 页码:15
通讯作者	Li, Ronggui([email protected])
摘要	Pine wilt disease (PWD) caused by Bursaphelenchus xylophilus is considered a major threat to pine forests worldwide. Uridine diphosphate (UDP)-glycosyltransferases (UGTs) catalyze the conjugation of small lipophilic compounds with sugars and play crucial roles in the detoxification and homeostatic processes in all living organisms. We investigated the molecular characteristics and biological functions of the gene UGT440A1 that encodes UGTs in B. xylophilus. The in situ hybridization results indicated that UGT440A1 is expressed in all developmental stages of B. xylophilus, particularly in the head, intestine, and hypodermis of the second-stage of juveniles (J2), third-stage of juveniles (J3) and fourth-stage of juveniles (J4) females and in almost the whole body of J4 males and adults. Recombinant UGT440A1 was observed mainly in the inclusion bodies, and the enzyme activity assay revealed that UGT440A1 could catalyze the glycosylation reaction of two types of flavonols (kaempferol and quercetin). RNA interference (RNAi) of UGT440A1 suppressed motility, feeding, and reproduction of B. xylophilus. Furthermore, UGT440A1 knockdown caused a delay in the development of PWD symptoms in the pine seedlings inoculated with the nematodes. These results suggest that UGT440A1 is involved in the pathogenic process of B. xylophilus and the information may facilitate a better understanding of the molecular mechanism of PWD.
关键词	Bursaphelenchus xylophilus UDP-glycosyltransferase motility reproduction pathogenicity
DOI	10.3389/fpls.2022.862594
收录类别	SCI
语种	英语
资助项目	Key Programs for Research and Development of Shandong Province, China[2016GNC110024]; National Natural Science Foundation of China[31070575]; National Natural Science Foundation of China[31901313]
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000810931300001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179534
专题	中国科学院海洋研究所
通讯作者	Li, Ronggui
作者单位	1.Qingdao Univ, Med Coll, Qingdao, Peoples R China 2.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China 3.Qingdao JiMo Peoples Hosp, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wang, Min,Du, Guicai,Fang, Junna,et al. UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus[J]. FRONTIERS IN PLANT SCIENCE,2022,13:15.
APA	Wang, Min.,Du, Guicai.,Fang, Junna.,Wang, Linsong.,Guo, Qunqun.,...&Li, Ronggui.(2022).UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus.FRONTIERS IN PLANT SCIENCE,13,15.
MLA	Wang, Min,et al."UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus".FRONTIERS IN PLANT SCIENCE 13(2022):15.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
fpls-13-862594.pdf（16142KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览