A self-powered rotating paper-based analytical device for sensing of thrombin

doi:10.1016/j.snb.2021.130917

	A self-powered rotating paper-based analytical device for sensing of thrombin
	Li, Qingling 1; Xu, Yuehan 1; Qi, Ji 2,5; Zheng, Xiaoli 1; Liu, Shudi 3; Lin, Dong 2,4; Zhang, Liangwei 2; Liu, Ping 1,2; Li, Bowei 2; Chen, Lingxin 2
	2022-01-15
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
卷号	351 页码:8
通讯作者	Li, Bowei([email protected]) ; Chen, Lingxin([email protected])
摘要	We firstly constructed a kind of self-powered rotating paper-based microfluidic chip and used it to detect thrombin. The integrated movable valve has the ability to control the fluid user-friendly on microfluidic paper-based analytical devices (mu PADs), and this advantage let it convenient to implement the complex multistep operations. In addition, this technique is compatible and versatile to the self-powered mu PADs at differing levels of complexity. To obtain the supercapacitor signal, the DNA sequence (DNA1) and a glucose oxidase (GOx) modified DNA sequence (GOx-DNA2) were linked with aptamer to form the sandwich structure. Then thrombin will combine with its specific aptamer by strong binding ability, and the GOx-DNA2 could be released and to catalyze the oxidation of glucose. Because of GOx-triggered reaction, the voltage can be generated to charge the paper supercapacitor and its signal could be monitored by digital multimeter. The assay shows good performance with wide linear range (3-1350 nM) and the limit of detection for the thrombin assay was down to 0.9 nM. The proposed rotating paper-based aptamer device can integrate the complex self-powered multistep operations, and show good potential to be applied for on-site assay in the field of diagnosis.
关键词	Microfluidic paper-based analytical devices MuPAD Biofuel cell Electrochemical Self-powered
DOI	10.1016/j.snb.2021.130917
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41776110]; National Natural Science Foundation of China[21675102]; National Natural Science Foundation of China[22106179]; National Natural Science Foundation of China[21701074]; Shandong Provincial Natural Science Foundation Key Project, China[ZR2020KB022]; Open Fund of CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences[KLMEES202002]
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000798024900001
出版者	ELSEVIER SCIENCE SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179387
专题	海洋生态与环境科学重点实验室
通讯作者	Li, Bowei; Chen, Lingxin
作者单位	1.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Collaborat Innovat Ctr Functionalized Probes Chem, Minist Educ,Key Lab Mol & Nano Probes,Inst Mol &, Jinan 250014, Peoples R China 2.Chinese Acad Sci, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China 4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li, Qingling,Xu, Yuehan,Qi, Ji,et al. A self-powered rotating paper-based analytical device for sensing of thrombin[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,351:8.
APA	Li, Qingling.,Xu, Yuehan.,Qi, Ji.,Zheng, Xiaoli.,Liu, Shudi.,...&Chen, Lingxin.(2022).A self-powered rotating paper-based analytical device for sensing of thrombin.SENSORS AND ACTUATORS B-CHEMICAL,351,8.
MLA	Li, Qingling,et al."A self-powered rotating paper-based analytical device for sensing of thrombin".SENSORS AND ACTUATORS B-CHEMICAL 351(2022):8.

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