Co-occurrence of Alexandrium minutum (Dinophyceae) ribotypes from the Chinese and Malaysian coastal waters and their toxin production

doi:10.1016/j.hal.2022.102238

	Co-occurrence of Alexandrium minutum (Dinophyceae) ribotypes from the Chinese and Malaysian coastal waters and their toxin production
	Liu, Minlu 1; Krock, Bernd 2; Yu, Rencheng 3; Leaw, Chui Pin 4; Lim, Po Teen 4; Ding, Guangmao 5; Wang, Na 1; Zheng, Jing 1; Gu, Haifeng 1,6,7
	2022-06-01
发表期刊	HARMFUL ALGAE
ISSN	1568-9883
卷号	115 页码:14
通讯作者	Gu, Haifeng([email protected])
摘要	The bloom-forming dinophyte Alexandrium minutum comprises biogeographic inferred, global and Pacific clades with both toxic and nontoxic strains reported. A. minutum has a wide distribution in the Western Pacific, but to date only a few strains have available DNA sequences. To fully understand its genetic diversity, sampling was undertaken from the Yellow Sea, the East and South China Sea, and five strains of A. minutum and two strains of its sister species, A. tamutum, were established. Their morphology was examined by light and scanning electron microscopy. In addition, sequences were obtained from both large subunit (LSU) ribosomal DNA and/or internal transcribed spacer (ITS) region. Strains of A. minutum are morphologically indistinguishable, characterized by a smaller cell size and a narrow sixth precingular plate. In contrast, A. tamutum has a wider sixth precingular plate. High nucleotide divergences of LSU (D1-D3) rDNA and ITS were revealed amongst strains of A. minutum (10% and 25%, respectively), and A. tamutum (3% and 13%, respectively). Molecular phylogenies based on LSU rDNA and ITS revealed three ribotypes (B-D) of A. minutum, and two ribotypes of A. tamutum in the Western Pacific. Seasonal sampling in the East China Sea to detect A. minutum using the DNA metabarcoding targeting ITS1 region was also performed. Our results showed that the ribotypes B and C of A. minutum co-occurred in the water. Paralytic shellfish toxin (PSTs) of all seven strains was analysed using liquid chromatography with tandem mass spectrometry (LC-MS/MS). PSTs were detected only in A. minutum ribotypes B and C with predominance of gonyautoxins 1/4. Our results suggest high diversity and risk potential of this toxic species in this region.
关键词	Cryptic species Dinoflagellates Harmful algal bloom (HAB) Metabarcoding Paralytic shellfish toxins (PSTs)
DOI	10.1016/j.hal.2022.102238
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42030404]; National Key Research and Development Program of China[2019YFE0124700]; Fund Project for Marine and Fishery Protection and Development in Fujian Province[FZJZ-2021-1]; Ministry of Higher Education of Malaysia HICOE; [LRGS/1/2020/UMT/01/1/3]
WOS研究方向	Marine & Freshwater Biology
WOS类目	Marine & Freshwater Biology
WOS记录号	WOS:000798742200001
出版者	ELSEVIER
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179384
专题	海洋生态与环境科学重点实验室
通讯作者	Gu, Haifeng
作者单位	1.Minist Nat Resources, Inst Oceanog 3, Xiamen 361005, Peoples R China 2.Helmholtz Zent Polar & Meeresforschung, Alfred Wegener Inst, Handelshafen 12, D-27570 Bremerhaven, Germany 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.Univ Malaya, Inst Ocean & Earth Sci, Bachok Marine Res Stn, Bachok 16310, Kelantan, Malaysia 5.Fishery Resources Monitoring Ctr Fujian Prov, Fuzhou 350003, Peoples R China 6.Minist Nat Resources, Key Lab Marine Ecol Conservat & Restorat, Xiamen 361005, Peoples R China 7.Fujian Prov Key Lab Marine Ecol Conservat & Restor, Xiamen 361005, Peoples R China
推荐引用方式 GB/T 7714	Liu, Minlu,Krock, Bernd,Yu, Rencheng,et al. Co-occurrence of Alexandrium minutum (Dinophyceae) ribotypes from the Chinese and Malaysian coastal waters and their toxin production[J]. HARMFUL ALGAE,2022,115:14.
APA	Liu, Minlu.,Krock, Bernd.,Yu, Rencheng.,Leaw, Chui Pin.,Lim, Po Teen.,...&Gu, Haifeng.(2022).Co-occurrence of Alexandrium minutum (Dinophyceae) ribotypes from the Chinese and Malaysian coastal waters and their toxin production.HARMFUL ALGAE,115,14.
MLA	Liu, Minlu,et al."Co-occurrence of Alexandrium minutum (Dinophyceae) ribotypes from the Chinese and Malaysian coastal waters and their toxin production".HARMFUL ALGAE 115(2022):14.

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