Genetic Diversity and Population Structure of Portunus trituberculatus in Released and Wild Populations Based on Microsatellite DNA Markers from the Yangtze Estuary

doi:10.3390/d14050374

	Genetic Diversity and Population Structure of Portunus trituberculatus in Released and Wild Populations Based on Microsatellite DNA Markers from the Yangtze Estuary
	Yue, Longtao1,2,3 ; Wang, Yibang1,2 ; Xian, Weiwei 1,2; Zhang, Hui1,2,3
	2022-05-01
发表期刊	DIVERSITY-BASEL
卷号	14 期号:5 页码:12
通讯作者	Xian, Weiwei([email protected]) ; Zhang, Hui([email protected])
摘要	Portunus trituberculatus is an important economic species of crab that is artificially bred and released in the Yangtze River Estuary and its adjacent sea areas. Based on six microsatellite markers, we investigate the genetic diversity and structure of 101 P. trituberculatus specimens collected from two hatcheries in Nantong and Zhoushan that participated in stock enhancement in the year 2019. We compared these with 124 wild specimens caught from 13 localities in the estuary. Analysis of several genetic diversity parameters (N-A, R-S, I, H-O, H-E, F-IS, and F-ST) for the 15 populations demonstrates that both released and wild populations possess relatively rich genetic diversity. Furthermore, the released groups demonstrate no less genetic variation between themselves than do the wild crabs. Most F-IS values are greater than zero, which shows inbreeding is common among specimens with geographically open sites. However, insufficient sampling may have led to a wide distribution of null alleles, a Hardy-Weinberg test disequilibrium in microsatellite markers PN22 and P04, and a lack of crab genetic diversity in site 14. All populations (except locality 14) have not suffered the bottleneck effect. Four subgroups can be seen to roughly spread longitudinally along the sample area by performing pairwise comparisons of genetic distance and F-ST values among the populations. No obvious topological heterogeneity is discovered among the four subgroups in a phylogenetic tree. The existence of genetic exchange and differentiation among the subgroups is also verified using structure analysis. Therefore, based on this evidence, we propose that the hatchery stock enhancements performed in Nantong and Zhoushan result in no reduction in genetic diversity for wild populations in the Yangtze Estuary in 2019.
关键词	P trituberculatus microsatellite loci stock enhancement effect evaluation genetic status hereditary constitution
DOI	10.3390/d14050374
收录类别	SCI
语种	英语
资助项目	Youth Innovation Promotion Association CAS[2020211]
WOS研究方向	Biodiversity & Conservation ; Environmental Sciences & Ecology
WOS类目	Biodiversity Conservation ; Ecology
WOS记录号	WOS:000803436600001
出版者	MDPI
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179354
专题	海洋生态与环境科学重点实验室
通讯作者	Xian, Weiwei; Zhang, Hui
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Yue, Longtao,Wang, Yibang,Xian, Weiwei,et al. Genetic Diversity and Population Structure of Portunus trituberculatus in Released and Wild Populations Based on Microsatellite DNA Markers from the Yangtze Estuary[J]. DIVERSITY-BASEL,2022,14(5):12.
APA	Yue, Longtao,Wang, Yibang,Xian, Weiwei,&Zhang, Hui.(2022).Genetic Diversity and Population Structure of Portunus trituberculatus in Released and Wild Populations Based on Microsatellite DNA Markers from the Yangtze Estuary.DIVERSITY-BASEL,14(5),12.
MLA	Yue, Longtao,et al."Genetic Diversity and Population Structure of Portunus trituberculatus in Released and Wild Populations Based on Microsatellite DNA Markers from the Yangtze Estuary".DIVERSITY-BASEL 14.5(2022):12.

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