Cultivation and Functional Characterization of a Deep-Sea Lentisphaerae Representative Reveals Its Unique Physiology and Ecology

doi:10.3389/fmars.2022.848136

IOCAS-IR > 实验海洋生物学重点实验室

	Cultivation and Functional Characterization of a Deep-Sea Lentisphaerae Representative Reveals Its Unique Physiology and Ecology
	Zhang, Tianhang 1,2,3,4; Zheng, Rikuan2,3,4 ; Liu, Rui 2,3,4; Li, Ronggui 1; Sun, Chaomin 2,3,4
	2022-04-14
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	9 页码:13
通讯作者	Li, Ronggui([email protected]) ; Sun, Chaomin([email protected])
摘要	As a member of Planctomycetes-Verrucomicrobia-Chlamydia (PVC) superphylum, the phylum Lentisphaerae is broadly distributed in various environments. Lentisphaerae members have been recognized as being typically difficult to isolate in pure culture-only five strains belonging to this phylum were cultured up to now-and therefore their major physiology and ecology are largely unknown. Here, using a polysaccharide degradation-driven strategy, we successfully cultured a representative of Lentisphaerae bacteria (Candidatus Coldseepensis marina zth2) from the deep-sea cold seep. Based on physiological and phylogenetic analyses, strain zth2 is identified as a type strain of a novel family. Notably, strain zth2 divides by a budding manner and a unique feature seen in ultrathin-section electron microscopic observation is the occurrence of narrow protrusions, namely, both cell wall and cytoplasm on the surface of strain zth2. Through growth assays and transcriptomic analyses, strain zth2 was verified to efficiently utilize polysaccharides, phosphate, and iron for growth. Strikingly, the utilization of polysaccharide, phosphate, and iron is correlative and jointly contributes to carbon, phosphorus, and iron metabolisms and thereby facilitating the production of energy. By mimicking the ocean environment and using operational taxonomic units (OTUs) analysis, we demonstrated that strain zth2 significantly enrich the microbial diversity by metabolizing polysaccharides or other substances. Overall, we propose that Lentisphaerae members are potential contributors to the cycling of nutrient, carbon, phosphorus, and other elements in the deep biosphere.
关键词	Lentisphaerae cold seep polysaccharide phosphorus cultivation deep-sea
DOI	10.3389/fmars.2022.848136
收录类别	SCI
语种	英语
资助项目	Key Deployment Projects of Center of Ocean Mega-Science of the Chinese Academy of Sciences[COMS2020Q04]; China Ocean Mineral Resources R&D Association Grant[DY135-B2-14]; Shandong Provincial Natural Science Foundation[ZR2021ZD28]; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301]; Major Research Plan of the National Natural Science Foundation[92051107]; Taishan Young Scholar Program of Shandong Province[tsqn20161051]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000796372300001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179231
专题	实验海洋生物学重点实验室深海极端环境与生命过程研究中心
通讯作者	Li, Ronggui; Sun, Chaomin
作者单位	1.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol &Center Deep Sea Res, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Tianhang,Zheng, Rikuan,Liu, Rui,et al. Cultivation and Functional Characterization of a Deep-Sea Lentisphaerae Representative Reveals Its Unique Physiology and Ecology[J]. FRONTIERS IN MARINE SCIENCE,2022,9:13.
APA	Zhang, Tianhang,Zheng, Rikuan,Liu, Rui,Li, Ronggui,&Sun, Chaomin.(2022).Cultivation and Functional Characterization of a Deep-Sea Lentisphaerae Representative Reveals Its Unique Physiology and Ecology.FRONTIERS IN MARINE SCIENCE,9,13.
MLA	Zhang, Tianhang,et al."Cultivation and Functional Characterization of a Deep-Sea Lentisphaerae Representative Reveals Its Unique Physiology and Ecology".FRONTIERS IN MARINE SCIENCE 9(2022):13.

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