A newly identified NLR-like gene participates in bacteria and virus infection possibly through regulating hemocytes apoptosis in shrimp

doi:10.1016/j.dci.2022.104395

IOCAS-IR > 实验海洋生物学重点实验室

	A newly identified NLR-like gene participates in bacteria and virus infection possibly through regulating hemocytes apoptosis in shrimp
	Li, Xuechun1,2,3,6 ; Li, Shihao 1,2,4,6; Sun, Mingzhe1,6 ; Yu, Yang1,2,4,6 ; Zhang, Xiaojun 1,2,4,6; Xiang, Jianhai1,2,6 ; Li, Fuhua 1,2,4,5,6
	2022-07-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	132 页码:10
通讯作者	Li, Shihao([email protected]) ; Li, Fuhua([email protected])
摘要	The nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) play important roles in innate immunity. Previously, we identified an NLR-like gene, LvNLRPL1, and found that it participated in Vibrio infection and regulated hemocytes apoptosis in the Pacific whiteleg shrimp Litopenaeus vannamei. However, it is still unclear whether other NLR-like genes exist in shrimp and how they function during virus infection. In the present study, a novel NLR-like gene (LvNLRPL2) was identified and functionally characterized in L. vannamei. LvNLRPL2 was highly expressed in hemocytes and responsive to both Vibrio parahaemolyticus and white spot syndrome virus (WSSV) infection. Knockdown of LvNLRPL2 could markedly increase the proliferation of Vibrio and the mortality of shrimp infected with V. parahaemolyticus, whereas inhibit in vivo WSSV propagation in shrimp, indicating its distinct roles during Vibrio and WSSV infection. After LvNLRPL2 knockdown, the apoptotic rate of hemocytes increased, and the expression levels of LvCaspase 2, 3 and 5 were significantly up-regulated. In addition, LvNLRPL2 could form a hetero-dimer with LvNLRPL1 through their NACHT domains. These results suggest that LvNLRPL2 might resist bacterial infection while promote WSSV propagation by forming heterodimer with LvNLRPL1 and then inhibiting apoptosis of hemocytes. These data will be helpful for understanding the functions of NLR-like genes and their regulation mechanisms in crustaceans.
关键词	NLRs Immune response Apoptosis Vibrio parahaemolyticus White spot syndrome virus
DOI	10.1016/j.dci.2022.104395
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31972829]; National Natural Science Foundation of China[31830100]; National Natural Science Foundation of China[41776158]; China Agriculture Research System of MOF and MARA
WOS研究方向	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS类目	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS记录号	WOS:000791225000001
出版者	ELSEVIER SCI LTD
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178997
专题	实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China 6.Chinese Acad Sci, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Xuechun,Li, Shihao,Sun, Mingzhe,et al. A newly identified NLR-like gene participates in bacteria and virus infection possibly through regulating hemocytes apoptosis in shrimp[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2022,132:10.
APA	Li, Xuechun.,Li, Shihao.,Sun, Mingzhe.,Yu, Yang.,Zhang, Xiaojun.,...&Li, Fuhua.(2022).A newly identified NLR-like gene participates in bacteria and virus infection possibly through regulating hemocytes apoptosis in shrimp.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,132,10.
MLA	Li, Xuechun,et al."A newly identified NLR-like gene participates in bacteria and virus infection possibly through regulating hemocytes apoptosis in shrimp".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 132(2022):10.

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