Characterization of vasa and dnd homologs in summer flounder, Paralichthys dentatus: Expression analysis and colocalization of PGCs during embryogenesis

doi:10.1016/j.theriogenology.2022.01.007

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization of vasa and dnd homologs in summer flounder, Paralichthys dentatus: Expression analysis and colocalization of PGCs during embryogenesis
	Du, Shuran 1,2,3,4; Zhou, Li 2,3,4,5; Wang, Xueying2,3,4 ; Xu, Shihong 2,3,4; Li, Jun 2,3,4; Song, Zongcheng 6; Liu, Qinghua 2,3,4
	2022-03-15
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	181 页码:180-189
通讯作者	Song, Zongcheng([email protected]) ; Liu, Qinghua([email protected])
摘要	Specification of primordial germ cells (PGCs) is particularly important for germline formation. Many maternal-effect genes such as vasa, dnd, and nanos have been identified. However, the research on distribution patterns of PGCs in marine fish is limited. Vasa has been widely used as a germ cell marker to identify its origination in teleosts because vasa RNA is a component of germ plasm. Dnd is known to be an RNA binding protein that protects germline-specific RNAs from degradation. In this study, we isolated full-length vasa and dnd cDNA from summer flounder to track germ cell origination and their expression patterns by RT-PCR and ISH. The results demonstrated that deduced amino acid sequence of Pdvas and Pddnd shared typically conserved motifs of their homologues and demonstrated high identities with other teleosts. Both vasa and dnd transcripts were exclusively detected in germ cells of the gonads. During embryogenesis, vasa and dnd RNA were located at the cleavage furrows of early cleavage stages, and then through proliferation and migration they eventually moved to a location at the predetermined genital ridge. Phylogenetic analysis revealed that summer flounder belongs to the Euteleostei species, but vasa/dnd transcripts localized at the cleavage furrows was similar to that in zebrafish (Osteriophysans). This suggests that germ cells differentiating at early embryogenesis have no direct relation with phylogenesis. At the same time, we found the spatio-temporal expression pattern of dnd was highly consistent with vasa during this process, which indicated the important function of dnd in keeping the target RNA from being degraded to maintain germ cell fate. These results will provide further understanding of germ plasm localization and PGC differentiation in teleosts, and facilitate germ cell manipulation in marine fishes. (c) 2022 Published by Elsevier Inc.
关键词	Summer flounder ( Paralichthys dentatus ) Vasa Dnd Germ cell localization
DOI	10.1016/j.theriogenology.2022.01.007
收录类别	SCI
语种	英语
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-4]; Shandong Province Natural Science Foundation[ZR2020KC038]; Research and Development Program of Shandong Province[2021LZGC029]; National Key Research and Development Program[2018YFD0901205]; National Key Research and Development Program[2018YFD0901204]; China Agriculture Research System[CARS-47]; National Marine Genetic Resource Center
WOS研究方向	Reproductive Biology ; Veterinary Sciences
WOS类目	Reproductive Biology ; Veterinary Sciences
WOS记录号	WOS:000788099500022
出版者	ELSEVIER SCIENCE INC
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178859
专题	实验海洋生物学重点实验室
通讯作者	Song, Zongcheng; Liu, Qinghua
作者单位	1.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China 2.Chinese Acad Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Ningde Normal Univ, Fujian Prov Univ, Coll Life Sci, Engn Res Ctr Mindong Aquat Prod Deep Proc, Ningde 352100, Peoples R China 6.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264319, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Du, Shuran,Zhou, Li,Wang, Xueying,et al. Characterization of vasa and dnd homologs in summer flounder, Paralichthys dentatus: Expression analysis and colocalization of PGCs during embryogenesis[J]. THERIOGENOLOGY,2022,181:180-189.
APA	Du, Shuran.,Zhou, Li.,Wang, Xueying.,Xu, Shihong.,Li, Jun.,...&Liu, Qinghua.(2022).Characterization of vasa and dnd homologs in summer flounder, Paralichthys dentatus: Expression analysis and colocalization of PGCs during embryogenesis.THERIOGENOLOGY,181,180-189.
MLA	Du, Shuran,et al."Characterization of vasa and dnd homologs in summer flounder, Paralichthys dentatus: Expression analysis and colocalization of PGCs during embryogenesis".THERIOGENOLOGY 181(2022):180-189.

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