Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)

doi:10.3389/fcimb.2022.875409

IOCAS-IR > 实验海洋生物学重点实验室

	Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)
	Li, Qian1 ; Chi, Heng 1,2; Shi, Xueyan 1; Gan, Qiujie 1; Dalmo, Roy Ambli 3; Sun, Yuan-yuan4 ; Tang, Xiaoqian 1,2; Xing, Jing 1,2; Sheng, Xiuzhen 1,2; Zhan, Wenbin 1,2
	2022-03-30
发表期刊	FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
ISSN	2235-2988
卷号	12 页码:11
通讯作者	Chi, Heng([email protected])
摘要	Adjuvants are used to increase the strength, quality, and duration of the immune response of vaccines. Neutrophils are the first immune cells that arrive at the injection site and can release DNA fibers together with granular proteins, so-called neutrophil extracellular traps (NETs), to entrap microbes in a sticky matrix of extracellular chromatin and microbicidal agents. Similar extracellular structures were also released by macrophages, mast cells, and eosinophils and are now generalized as "ETs." Here we demonstrated that Alum adjuvant stimulation led to peritoneal cells swarming and ET release in vitro. Moreover, compared to antigen stimulation alone, ET release was significantly increased after stimulation with antigen-mixed adjuvants and in a time- and dose-dependent manner. In vivo, we were able to monitor and quantify the continuous changes of the ET release in the same fish by using the small animal in vivo imaging instrument at different times during the early stages after intraperitoneal immunization. The results showed that the fluorescence signal of ETs in the peritoneum increased from 0 to 12 h after injection and then gradually decreased. The fluorescence signals came from extracellular DNA fibers, which are sensitive to DNase I and confirmed by microscopy of peritoneal fluid ex vivo. In summary, this study introduced a new method for detecting ETs in the peritoneum of fish in vivo and indicated that ET formation is involved in the immune response at the early stage after intraperitoneal immunization to vaccines.
关键词	extracellular traps (ETs) antigen adjuvant vaccination fish
DOI	10.3389/fcimb.2022.875409
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31872594]; National Natural Science Foundation of China[31730101]; National Key Research and Development Program of China[2019YFD0900101]; National Key Research and Development Program of China[2019YFD0900102]
WOS研究方向	Immunology ; Microbiology
WOS类目	Immunology ; Microbiology
WOS记录号	WOS:000784019200001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178778
专题	实验海洋生物学重点实验室
通讯作者	Chi, Heng
作者单位	1.Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China 3.Univ Tromso, Arctic Univ Norway, Norwegian Coll Fishery Sci, Fac Biosci Fisheries & Econ, Tromso, Norway 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Qian,Chi, Heng,Shi, Xueyan,et al. Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2022,12:11.
APA	Li, Qian.,Chi, Heng.,Shi, Xueyan.,Gan, Qiujie.,Dalmo, Roy Ambli.,...&Zhan, Wenbin.(2022).Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus).FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,12,11.
MLA	Li, Qian,et al."Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 12(2022):11.

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