Highly Sensitive and Specific Responses of Oyster Hemocytes to Copper Exposure: Single-Cell Transcriptomic Analysis of Different Cell Populations

doi:10.1021/acs.est.1c07510

IOCAS-IR

	Highly Sensitive and Specific Responses of Oyster Hemocytes to Copper Exposure: Single-Cell Transcriptomic Analysis of Different Cell Populations
	Meng, Jie1,2,3 ; Wang, Wen-Xiong 1,4,5,6
	2022-02-15
发表期刊	ENVIRONMENTAL SCIENCE & TECHNOLOGY
ISSN	0013-936X
卷号	56 期号:4 页码:2497-2510
通讯作者	Wang, Wen-Xiong([email protected])
摘要	Oyster hemocytes are the primary vehicles transporting and detoxifying metals and are regarded as important cells for the occurrence of colored oysters due to copper (Cu) contamination. However, its heterogeneous responses under Cu exposure have not been studied. Single-cell transcriptome profiling (scRNA-seq) provides high-resolution visual insights into tissue dynamics and environmental responses. Here, we used scRNA-seq to study the responses of different cell populations of hemocytes under Cu exposure in an estuarine oyster Crassostrea hongkongensis. The 1900 population-specific Cu-responsive genes were identified in 12 clusters of hemocytes, which provided a more sensitive technique for examining Cu exposure. The granulocyte, semi-granulocyte, and hyalinocyte had specific responses, while the granulocyte was the most important responsive cell type and displayed heterogeneity responses of its two subtypes. In one subtype, Cu was transported with metal transporters and chelated with Cu chaperons in the cytoplasm. Excess Cu disturbed oxidative phosphorylation and induced reactive oxygen species production. However, in the other subtype, endocytosis was mainly responsible for Cu internalization, which was sequestered in membrane-bound granules. Collectively, our results provided the first mRNA expression profile of hemocytes in oysters and revealed the heterogeneity responses under Cu exposure.
关键词	oyster Cu exposure scRNA-seq granulocytes heterogeneity responses
DOI	10.1021/acs.est.1c07510
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[32102815]; Youth Innovation Promotion Association of the Chinese Academy of Science[2019210]
WOS研究方向	Engineering ; Environmental Sciences & Ecology
WOS类目	Engineering, Environmental ; Environmental Sciences
WOS记录号	WOS:000763099700040
出版者	AMER CHEMICAL SOC
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178438
专题	中国科学院海洋研究所
通讯作者	Wang, Wen-Xiong
作者单位	1.Southern Marine Sci & Engn Guangdong Lab Guangzho, Guangzhou 511458, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.City Univ Hong Kong, Sch Energy & Environm, Hong Kong 999077, Peoples R China 5.City Univ Hong Kong, State Key Lab Marine Pollut, Hong Kong 999077, Peoples R China 6.City Univ Hong Kong, Res Ctr Oceans & Human Hlth, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Meng, Jie,Wang, Wen-Xiong. Highly Sensitive and Specific Responses of Oyster Hemocytes to Copper Exposure: Single-Cell Transcriptomic Analysis of Different Cell Populations[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2022,56(4):2497-2510.
APA	Meng, Jie,&Wang, Wen-Xiong.(2022).Highly Sensitive and Specific Responses of Oyster Hemocytes to Copper Exposure: Single-Cell Transcriptomic Analysis of Different Cell Populations.ENVIRONMENTAL SCIENCE & TECHNOLOGY,56(4),2497-2510.
MLA	Meng, Jie,et al."Highly Sensitive and Specific Responses of Oyster Hemocytes to Copper Exposure: Single-Cell Transcriptomic Analysis of Different Cell Populations".ENVIRONMENTAL SCIENCE & TECHNOLOGY 56.4(2022):2497-2510.

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