Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity

doi:10.3389/fimmu.2022.813173

IOCAS-IR > 实验海洋生物学重点实验室

	Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity
	Wu, Meng 1,2,3,4; Jia, Bei-bei 1,2,3,4; Li, Mo-fei1,2,3
	2022-02-25
发表期刊	FRONTIERS IN IMMUNOLOGY
ISSN	1664-3224
卷号	13 页码:14
通讯作者	Li, Mo-fei([email protected])
摘要	In the complement system, C3 is a central component in complement activation, immune defense and immune regulation. In all pathways of complement activation, the pivotal step is conversion of the component C3 to C3b and C3a, which is responsible to eliminate the pathogen and opsonization. In this study, we examined the immunological properties of C3 and its activated fragment C3a from Japanese flounder (Paralichthys olivaceus) (PoC3 and PoC3a), a teleost species with important economic value. PoC3 is composed of 1655 amino acid residues, contains the six domains and highly conserved GCGEQ sequence of the C3 family. We found that PoC3 expression occurred in nine different tissues and was upregulated by bacterial challenge. In serum, PoC3 was able to bind to a broad-spectrum of bacteria, and purified native PoC3 could directly kill specific pathogen. When PoC3 expression in Japanese flounder was knocked down by siRNA, serum complement activity was significantly decreased, and bacterial replication in fish tissues was significantly increased. Recombinant PoC3a (rPoC3a) exhibited apparent binding capacities to bacteria and Japanese flounder peripheral blood leukocytes (PBL) and induce chemotaxis of PBL. Japanese flounder administered rPoC3a exhibited enhanced resistance against bacterial infection. Taken together, these results indicate that PoC3 is likely a key factor of complement activation, and PoC3 and PoC3a are required for optimal defense against bacterial infection in teleost.
关键词	C3 C3a complement Japanese flounder bacterial infection
DOI	10.3389/fimmu.2022.813173
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31972831]; National Key Research and Development Program of China[2018YFD0900501]; Taishan Scholar Program of Shandong Province
WOS研究方向	Immunology
WOS类目	Immunology
WOS记录号	WOS:000767597600001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178174
专题	实验海洋生物学重点实验室
通讯作者	Li, Mo-fei
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Inst Oceanol, Ctr Ocean Mega Sci, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wu, Meng,Jia, Bei-bei,Li, Mo-fei. Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity[J]. FRONTIERS IN IMMUNOLOGY,2022,13:14.
APA	Wu, Meng,Jia, Bei-bei,&Li, Mo-fei.(2022).Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity.FRONTIERS IN IMMUNOLOGY,13,14.
MLA	Wu, Meng,et al."Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity".FRONTIERS IN IMMUNOLOGY 13(2022):14.

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