A Novel TRIM9 Protein Promotes NF-kappa B Activation Through Interacting With LvIMD in Shrimp During WSSV Infection

doi:10.3389/fimmu.2022.819881

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	A Novel TRIM9 Protein Promotes NF-kappa B Activation Through Interacting With LvIMD in Shrimp During WSSV Infection
	Sun, Mingzhe1,2 ; Li, Shihao 1,2,3,4; Jin, Songjun 1,2; Li, Xuechun5 ; Xiang, Jianhai1,2,3 ; Li, Fuhua 1,2,3,4,6
	2022-02-23
发表期刊	FRONTIERS IN IMMUNOLOGY
ISSN	1664-3224
卷号	13 页码:12
通讯作者	Li, Shihao([email protected]) ; Li, Fuhua([email protected])
摘要	The TRIpartite Motif (TRIM) proteins play key roles in cell differentiation, apoptosis, development, autophagy, and innate immunity in vertebrates. In the present study, a novel TRIM9 homolog (designated as LvTRIM9-1) specifically expressed in the lymphoid organ of shrimp was identified from the Pacific whiteleg shrimp Litopenaeus vannamei. Its deduced amino acid sequence possesses the typical features of TRIM proteins, including a RING domain, two B-boxes, a coiled-coil domain, a FN3 domain, and a SPRY domain. The transcripts of LvTRIM9-1 were mainly located in the lymphoid tubules of the lymphoid organ. Knockdown of LvTRIM9-1 could apparently inhibit the transcriptions of some genes from white spot syndrome virus (WSSV) and reduce the viral propagation in the lymphoid organ. Overexpression of LvTRIM9-1 in mammalian cells could activate the promoter activity of NF-kappa B, and an in vivo experiment in shrimp showed that knockdown of LvTRIM9-1 reduced the expression of LvRelish in the lymphoid organ. Yeast two-hybridization and co-immunoprecipitation (Co-IP) assays confirmed that LvTRIM9-1 could directly interact with LvIMD, a key component of the IMD pathway, through its SPRY domain. These data suggest that LvTRIM9-1 could activate the IMD pathway in shrimp via interaction with LvIMD. This is the first evidence to show the regulation of a TRIM9 protein on the IMD pathway through its direct interaction with IMD, which will enrich our knowledge on the role of TRIM proteins in innate immunity of invertebrates.
关键词	TRIM9 lymphoid organ IMD signaling pathway WSSV Litopenaeus vannamei
DOI	10.3389/fimmu.2022.819881
收录类别	SCI
语种	英语
WOS研究方向	Immunology
WOS类目	Immunology
WOS记录号	WOS:000766533800001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178144
专题	实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, Mingzhe,Li, Shihao,Jin, Songjun,et al. A Novel TRIM9 Protein Promotes NF-kappa B Activation Through Interacting With LvIMD in Shrimp During WSSV Infection[J]. FRONTIERS IN IMMUNOLOGY,2022,13:12.
APA	Sun, Mingzhe,Li, Shihao,Jin, Songjun,Li, Xuechun,Xiang, Jianhai,&Li, Fuhua.(2022).A Novel TRIM9 Protein Promotes NF-kappa B Activation Through Interacting With LvIMD in Shrimp During WSSV Infection.FRONTIERS IN IMMUNOLOGY,13,12.
MLA	Sun, Mingzhe,et al."A Novel TRIM9 Protein Promotes NF-kappa B Activation Through Interacting With LvIMD in Shrimp During WSSV Infection".FRONTIERS IN IMMUNOLOGY 13(2022):12.

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