Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions

doi:10.3389/fgene.2021.795706

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	Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions
	Chan, Jiulin 1,2,3,4; Wang, Lu1,2,3,4,5 ; Li, Li 1,2,3,4,5; Mu, Kang1,2,3,4,5 ; Bushek, David 6; Xu, Yue 1,2,3,4; Guo, Ximing 6; Zhang, Guofan 1,2,3,4,5; Zhang, Linlin 1,2,3,4,5
	2021-12-03
发表期刊	FRONTIERS IN GENETICS
卷号	12 页码:16
通讯作者	Guo, Ximing([email protected]) ; Zhang, Guofan([email protected]) ; Zhang, Linlin([email protected])
摘要	Infectious disease outbreaks are causing widespread declines of marine invertebrates including corals, sea stars, shrimps, and molluscs. Dermo is a lethal infectious disease of the eastern oyster Crassostrea virginica caused by the protist Perkinsus marinus. The Pacific oyster Crassostrea gigas is resistant to Dermo due to differences in the host-parasite interaction that is not well understood. We compared transcriptomic responses to P. marinus challenge in the two oysters at early and late infection stages. Dynamic and orchestrated regulation of large sets of innate immune response genes were observed in both species with remarkably similar patterns for most orthologs, although responses in C. virginica were stronger, suggesting strong or over-reacting immune response could be a cause of host mortality. Between the two species, several key immune response gene families differed in their expansion, sequence variation and/or transcriptional response to P. marinus, reflecting evolutionary divergence in host-parasite interaction. Of note, significant upregulation of inhibitors of apoptosis (IAPs) was observed in resistant C. gigas but not in susceptible C. virginica, suggesting upregulation of IAPs is an active defense mechanism, not a passive response orchestrated by P. marinus. Compared with C. gigas, C. virginica exhibited greater expansion of toll-like receptors (TLRs) and positive selection in P. marinus responsive TLRs. The C1q domain containing proteins (C1qDCs) with the galactose-binding lectin domain that is involved in P. marinus recognition, were only present and significantly upregulated in C. virginica. These results point to previously undescribed differences in host defense genes between the two oyster species that may account for the difference in susceptibility, providing an expanded portrait of the evolutionary dynamics of host-parasite interaction in lophotrochozoans that lack adaptive immunity. Our findings suggest that C. virginica and P. marinus have a history of coevolution and the recent outbreaks may be due to increased virulence of the parasite.
关键词	oyster comparative transcriptomics dermo disease innate immune response host-parasite interaction gene expansion adaptation
DOI	10.3389/fgene.2021.795706
收录类别	SCI
语种	英语
资助项目	Priority Research Program of the Chinese Academy of Sciences[XDB42000000]; National Natural Science Foundation of China[41976088]; Key Development Project of Centre for Ocean Mega-Research of Science, Chinese academy of science[COMS2019R01]; USDA NIFA Hatch project[1009201-NJ32114]; USDA NIFA Hatch/Animal Health project[1004475-NJ32920]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000761070300001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178063
专题	实验海洋生物学重点实验室
通讯作者	Guo, Ximing; Zhang, Guofan; Zhang, Linlin
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Univ Chinese Acad Sci, Coll Marine Sci, Beijing, Peoples R China 6.Rutgers State Univ, Dept Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Chan, Jiulin,Wang, Lu,Li, Li,et al. Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions[J]. FRONTIERS IN GENETICS,2021,12:16.
APA	Chan, Jiulin.,Wang, Lu.,Li, Li.,Mu, Kang.,Bushek, David.,...&Zhang, Linlin.(2021).Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions.FRONTIERS IN GENETICS,12,16.
MLA	Chan, Jiulin,et al."Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions".FRONTIERS IN GENETICS 12(2021):16.

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