Synthesis of Abscisic Acid in Neopyropia yezoensis and Its Regulation of Antioxidase Genes Expressions Under Hypersaline Stress

doi:10.3389/fmicb.2021.775710

IOCAS-IR > 实验海洋生物学重点实验室

	Synthesis of Abscisic Acid in Neopyropia yezoensis and Its Regulation of Antioxidase Genes Expressions Under Hypersaline Stress
	Yang, Jiali1,3 ; Gu, Wenhui 1,2; Feng, Zezhong 1,4; Yu, Bin 1,3; Niu, Jianfeng 1,2; Wang, Guangce 1,2
	2022-01-10
发表期刊	FRONTIERS IN MICROBIOLOGY
卷号	12 页码:15
通讯作者	Niu, Jianfeng([email protected]) ; Wang, Guangce([email protected])
摘要	Abscisic acid (ABA) is regarded as crucial for plant adaptation to water-limited conditions and it functions evolutionarily conserved. Thus, insights into the synthesis of ABA and its regulation on downstream stress-responsive genes in Neopyropia yezoensis, a typical Archaeplastida distributed in intertidal zone, will improve the knowledge about how ABA signaling evolved in plants. Here, the variations in ABA contents, antioxidant enzyme activities and expression of the target genes were determined under the presence of exogenous ABA and two specific inhibitors of the ABA precursor synthesis. ABA content was down-regulated under the treatments of each or the combination of the two inhibitors. Antioxidant enzyme activities like SOD, CAT and APX were decreased slightly with inhibitors, but up-regulated when the addition of exogenous ABA. The quantitative assays using real-time PCR (qRT-PCR) results were consistent with the enzyme activities. All the results suggested that ABA can also alleviate oxidative stress in N. yezoensis as it in terrestrial plant. Combined with the transcriptome assay, it was hypothesized that ABA is synthesized in N. yezoensis via a pathway that is similar to the carotenoid pathway in higher plants, and both the MVA and that the MEP pathways for isoprenyl pyrophosphate (IPP) synthesis likely exist simultaneously. The ABA signaling pathway in N. yezoensis was also analyzed from an evolutionary standpoint and it was illustrated that the emergence of the ABA signaling pathway in this alga is an ancestral one. In addition, the presence of the ABRE motif in the promoter region of antioxidase genes suggested that the antioxidase system is regulated by the ABA signaling pathway.
关键词	abscisic acid antioxidase ABRE motif ABA signaling pathway Neopyropia yezoensis
DOI	10.3389/fmicb.2021.775710
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41776150]; National Natural Science Foundation of China[41476140]; China Agriculture Research System of MOF; Key Deployment Project of the Centre for Ocean Mega-Research of Science; Chinese Academy of Sciences[COMS2019Q02]; Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2019JZZY010815]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000748092600001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177942
专题	实验海洋生物学重点实验室
通讯作者	Niu, Jianfeng; Wang, Guangce
作者单位	1.Chinese Acad Sci IOCAS, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China 4.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yang, Jiali,Gu, Wenhui,Feng, Zezhong,et al. Synthesis of Abscisic Acid in Neopyropia yezoensis and Its Regulation of Antioxidase Genes Expressions Under Hypersaline Stress[J]. FRONTIERS IN MICROBIOLOGY,2022,12:15.
APA	Yang, Jiali,Gu, Wenhui,Feng, Zezhong,Yu, Bin,Niu, Jianfeng,&Wang, Guangce.(2022).Synthesis of Abscisic Acid in Neopyropia yezoensis and Its Regulation of Antioxidase Genes Expressions Under Hypersaline Stress.FRONTIERS IN MICROBIOLOGY,12,15.
MLA	Yang, Jiali,et al."Synthesis of Abscisic Acid in Neopyropia yezoensis and Its Regulation of Antioxidase Genes Expressions Under Hypersaline Stress".FRONTIERS IN MICROBIOLOGY 12(2022):15.

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