Emerging roles of circRNAs in regulating thermal and hypoxic stresses in Apostichopus japonicus (Echinodermata: Holothuroidea)

doi:10.1016/j.ecoenv.2021.112994

	Emerging roles of circRNAs in regulating thermal and hypoxic stresses in Apostichopus japonicus (Echinodermata: Holothuroidea)
	Huo, Da 1,2,3,4,6; Sun, Lina 1,2,3,4,5,6; Sun, Jingchun1,2,3,4,6 ; Lin, Chenggang 1,2,3,4,6; Liu, Shilin 1,2,3,4,6; Zhang, Libin 1,2,3,4,5,6; Yang, Hongsheng1,2,3,4,5,6,7
	2021-12-25
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
卷号	228 页码:9
通讯作者	Sun, Lina([email protected])
摘要	Some sea cucumbers are economically and ecologically important, but they are threatened by thermal and hypoxic stress in changing oceanographic conditions. We construct circRNAs profiles, reveal circRNAs characters, and illustrate the potential regulatory roles of circRNAs in one commercially important species of sea cucumber, Apostichopus japonicus. Reads are distributed in intergenic (44.14%), exonic (48.26%) and intronic (7.60%) regions of the genome. A total of 1684 circRNAs were identified, and the most common spliced length is 269 nt in the present study. In three treatments (HT [thermal stress], LO [hypoxic stress], and HL [combined thermal and hypoxic stress]), 24, 27 and 27 differentially expressed (DE) circRNAs were identified, respectively. Five novel DE-circRNAs commonly occur in these treatments (novel_circ_0003311, novel_circ_0000229, novel_circ_0003944, novel_circ_0001458 and novel_circ_0000707), and based on them, potential circRNA-miRNA binding pairs were predicted. Sanger sequencing, RNase R treatment experiment and qPCR validation identified the accuracy of the circRNAs. Key circRNAs identified in the present study were covalently closed and were more stable under RNase R treatment than linear RNAs. Based on function analysis, circRNAs could regulate metabolic process, signal transduction, and ion responses in A. japonicus when exposed to thermal and hypoxic stress, and 'regulation of response to stimulus' is a common gene ontology (GO) term that is significantly enriched in each treatment; GO terms for 'DNA' and 'stress' are commonly enriched in heat-related treatments (HT and HL); and GO terms for 'protein' are commonly enriched in hypoxia-related treatments (LO and HL). When environmentally stressed, 'metabolism,' 'transport and catabolism,' 'membrane transport,' and 'signal transduction' were significantly responded in sea cucumber based on KEGG analysis. We provide insights into circRNA functions in stress regulation and lay a foundation for invertebrate circRNA research.
关键词	Circular RNA Echinoderm Heat Anoxia Environmental stress
DOI	10.1016/j.ecoenv.2021.112994
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030304]; National Natural Science Foundation of China[42106106]; National Natural Science Foundation of China[42030408]; China Postdoctoral Science Foundation[2020M682247]; Postdoctoral Innovative Talents Support Program of Shandong Province[SDBX2020006]; Major Scientific and Technological Innovation Projects in Shandong Province[2019JZZY010812]; Shandong Provincial Natural Science Foundation[ZR202102200540]; Special Research Assistant Project of Chinese Academy of Sciences, Qingdao Postdoctoral Applied Research Project[2020170]; Youth Innovation Promotion Association CAS[2019209]
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
WOS类目	Environmental Sciences ; Toxicology
WOS记录号	WOS:000744241900006
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177911
专题	海洋生态与环境科学重点实验室
通讯作者	Sun, Lina
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Chinese Acad Sci, Innovat Seed Design, Wuhan 430071, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Huo, Da,Sun, Lina,Sun, Jingchun,et al. Emerging roles of circRNAs in regulating thermal and hypoxic stresses in Apostichopus japonicus (Echinodermata: Holothuroidea)[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,228:9.
APA	Huo, Da.,Sun, Lina.,Sun, Jingchun.,Lin, Chenggang.,Liu, Shilin.,...&Yang, Hongsheng.(2021).Emerging roles of circRNAs in regulating thermal and hypoxic stresses in Apostichopus japonicus (Echinodermata: Holothuroidea).ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,228,9.
MLA	Huo, Da,et al."Emerging roles of circRNAs in regulating thermal and hypoxic stresses in Apostichopus japonicus (Echinodermata: Holothuroidea)".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 228(2021):9.

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