Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus

doi:10.1007/s00343-021-1030-0

	Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus
	Zhang, Shufeng 1; Yuan, Chunjuan 1; Chen, Ying 1; Lin, Lin 1; Wang, Dazhi 1,2
	2021-12-27
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
页码	14
通讯作者	Wang, Dazhi([email protected])
摘要	Dinoflagellates are the major causative agents of harmful algal blooms in the global ocean and they usually form blooms under conditions of very low dissolved inorganic phosphorus (DIP). However, the mechanisms underpinning the dinoflagellate blooms remain unclear. Here, we quantitatively compared protein expression profiles of a marine dinoflagellate, Prorocentrum donghaiense, grown in inorganic P-replete, P-deficient, and DIP- and dissolved organic phosphorus (DOP)-resupplied conditions by employing a Tandem Mass Tag (TMT)-based quantitative proteomic approach. Proteins involved in intracellular P reallocation, organic P, and non-P lipid utilization were up-regulated under the P-deficient condition, while inorganic phosphate transporters varied insignificantly. In response to the P resupplementation, nitrogen metabolism, ribosome, porphyrin, and chlorophyll metabolism were up-regulated, while lysosome, and starch and sucrose metabolism were down-regulated. Notably, photosynthesis was up-regulated and secondary metabolism was down-regulated only in the DIP-resupplied cells, whereas amino acid metabolism and vitamin B6 metabolism were up-regulated in the DOP-resupplied cells, indicating differential response mechanisms of P. donghaiense to DIP or DOP resupplementation. Our results indicated that P. donghaiense initiated multiple strategies in response to an ambient inorganic P-deficiency, and its efficient DOP assimilation by providing both P and carbon sources might be a key factor driving bloom formations of P. donghaiense in a low DIP environment.
关键词	marine dinoflagellates harmful algal blooms Prorocentrum donghaiense phosphorus quantitative proteomics
DOI	10.1007/s00343-021-1030-0
收录类别	SCI
语种	英语
资助项目	National Key Research Development Program of China[2017YFC1404302]; National Natural Science Foundation of China[41425021]; National Natural Science Foundation of China[41706131]; Open Fund of CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences[KLMEES201806]; Ten-Thousand Talents Program for leading talents in science and technological innovation
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000734749000006
出版者	SCIENCE PRESS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177642
专题	海洋生态与环境科学重点实验室
通讯作者	Wang, Dazhi
作者单位	1.Xiamen Univ, Coll Environm & Ecol, State Key Lab Marine Environm Sci, Xiamen 361102, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Shufeng,Yuan, Chunjuan,Chen, Ying,et al. Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2021:14.
APA	Zhang, Shufeng,Yuan, Chunjuan,Chen, Ying,Lin, Lin,&Wang, Dazhi.(2021).Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,14.
MLA	Zhang, Shufeng,et al."Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2021):14.

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