Refinement and Neutralization Evaluation of the F(ab')(2) Type of Antivenom against the Deadly Jellyfish Nemopilema nomurai Toxins

doi:10.3390/ijms222312672

IOCAS-IR > 实验海洋生物学重点实验室

	Refinement and Neutralization Evaluation of the F(ab')(2) Type of Antivenom against the Deadly Jellyfish Nemopilema nomurai Toxins
	Li, Rongfeng 1,2; Yu, Huahua1,2 ; Li, Aoyu1,3 ; Yu, Chunlin1,3 ; Li, Pengcheng1,2
	2021-12-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	22 期号:23 页码:13
通讯作者	Li, Rongfeng([email protected]) ; Li, Pengcheng([email protected])
摘要	Jellyfish stings threaten people's health and even life in coastal areas worldwide. Nemopilema nomurai is one of the most dangerous jellyfish in the East Asian Marginal Seas, which not only stings hundreds of thousands of people every year but also is assumed to be responsible for most deaths by jellyfish stings in China. However, there is no effective first-aid drug, such as antivenoms, for the treatment of severe stings by N. nomurai to date. In this study, we prepared a N. nomurai antiserum from rabbits using inactivated N. nomurai toxins (NnTXs) and isolated the IgG type of antivenom (IgG-AntiNnTXs) from the antiserum. Subsequently, IgG-AntiNnTXs were refined with multiple optimizations to remove Fc fragments. Finally, the F(ab')(2) type of antivenom (F(ab')(2)-AntiNnTXs) was purified using Superdex 200 and protein A columns. The neutralization efficacy of both types of antivenom was analyzed in vitro and in vivo, and the results showed that both IgG and F(ab')(2) types of antivenom have some neutralization effect on the metalloproteinase activity of NnTXs in vitro and could also decrease the mortality of mice in the first 4 h after injection. This study provides some useful information for the development of an effective antivenom for N. nomurai stings in the future.
关键词	jellyfish Nemopilema nomurai antivenom NnTXs IgG-AntiNnTXs F(ab')(2)-AntiNnTXs
DOI	10.3390/ijms222312672
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41876164]; National Natural Science Foundation of China[41776163]; Natural Science Foundation of Shandong Province[ZR2019QD012]; National Key Research and Development Program of China[2017YFE0111100-04]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000734854200001
出版者	MDPI
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177630
专题	实验海洋生物学重点实验室
通讯作者	Li, Rongfeng; Li, Pengcheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Rongfeng,Yu, Huahua,Li, Aoyu,et al. Refinement and Neutralization Evaluation of the F(ab')(2) Type of Antivenom against the Deadly Jellyfish Nemopilema nomurai Toxins[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(23):13.
APA	Li, Rongfeng,Yu, Huahua,Li, Aoyu,Yu, Chunlin,&Li, Pengcheng.(2021).Refinement and Neutralization Evaluation of the F(ab')(2) Type of Antivenom against the Deadly Jellyfish Nemopilema nomurai Toxins.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(23),13.
MLA	Li, Rongfeng,et al."Refinement and Neutralization Evaluation of the F(ab')(2) Type of Antivenom against the Deadly Jellyfish Nemopilema nomurai Toxins".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.23(2021):13.