A strategy for promoting carbon flux into fatty acid and astaxanthin biosynthesis by inhibiting the alternative oxidase respiratory pathway in Haematococcus pluvialis

doi:10.1016/j.biortech.2021.126275

IOCAS-IR > 实验海洋生物学重点实验室

	A strategy for promoting carbon flux into fatty acid and astaxanthin biosynthesis by inhibiting the alternative oxidase respiratory pathway in Haematococcus pluvialis
	Zhang, Litao 1,2,3; Zhang, Chunhui1,2 ; Xu, Ran 1,2; Yu, Wenjie1,2 ; Liu, Jianguo 1,2,3,4
	2022
发表期刊	BIORESOURCE TECHNOLOGY
ISSN	0960-8524
卷号	344 页码:9
通讯作者	Liu, Jianguo([email protected])
摘要	In the present study, the mechanisms for facilitating fatty acid and astaxanthin biosynthesis-related processes by inhibiting the alternative oxidase (AOX) respiratory pathway in Haematococcus pluvialis was investigated. The restriction of the AOX pathway induced the accumulation of reactive oxygen species, NAD(P)H and its substrates (acetyl-CoA, pyruvate and glyceraldehy-3-phosphate), which are required for fatty acid and astaxanthin production, thereby promoting the carbon flux into fatty acid and astaxanthin biosynthesis. During a 9-day incubation period, the fatty acid and astaxanthin contents increased by 20.6% and 20.7%, respectively, when the AOX pathway was inhibited approximately 37.7%. The AOX pathway may be inhibited by nutrient (nitrogen and phosphorus) removal, inhibitor addition and air/CO2 aeration adjustments in the large-scale cultivation of H. pluvialis. Therefore, the current study provides a useful enhancement strategy for fatty acid and astaxanthin coproduction and elucidates the roles of the AOX pathway in regulating fatty acid and astaxanthin biosynthesis.
关键词	Alternative oxidase Astaxanthin Fatty acid Haematococcus pluvialis Stimulator
DOI	10.1016/j.biortech.2021.126275
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFE0107200]; National Natural Science Foundation of China[31872588]; National Natural Science Foundation of China[U1706209]; Major Achievement Support Plan Project of LMBB[M019NO01]; Shandong Provincial Natural Science Foundation, China[ZR2019BC057]
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:000732830900011
出版者	ELSEVIER SCI LTD
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177573
专题	实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Qingdao Langyatai Grp Co Ltd, Shandong Engn Technol Collaborat Innovat Ctr Edib, Qingdao 266400, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Zhang, Litao,Zhang, Chunhui,Xu, Ran,et al. A strategy for promoting carbon flux into fatty acid and astaxanthin biosynthesis by inhibiting the alternative oxidase respiratory pathway in Haematococcus pluvialis[J]. BIORESOURCE TECHNOLOGY,2022,344:9.
APA	Zhang, Litao,Zhang, Chunhui,Xu, Ran,Yu, Wenjie,&Liu, Jianguo.(2022).A strategy for promoting carbon flux into fatty acid and astaxanthin biosynthesis by inhibiting the alternative oxidase respiratory pathway in Haematococcus pluvialis.BIORESOURCE TECHNOLOGY,344,9.
MLA	Zhang, Litao,et al."A strategy for promoting carbon flux into fatty acid and astaxanthin biosynthesis by inhibiting the alternative oxidase respiratory pathway in Haematococcus pluvialis".BIORESOURCE TECHNOLOGY 344(2022):9.

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