Chlorophyll fluorescence as a light signal enhances iron uptake by the marine diatom Phaeodactylum tricornutum under high-cell density conditions

doi:10.1186/s12915-021-01177-z

IOCAS-IR > 实验海洋生物学重点实验室

	Chlorophyll fluorescence as a light signal enhances iron uptake by the marine diatom Phaeodactylum tricornutum under high-cell density conditions
	Liu, Xuehua 1,2,3,4; Xie, Xiujun 1,2,3; Gao, Shan 1,2,3; Wang, Lepu 1,2,3,4; Zhou, Lu 1,2,3,4; Liu, Yao 5; Hu, Qiang 6; Gu, Wenhui 1,2,3; Wang, Guangce 1,2,3
	2021-11-23
发表期刊	BMC BIOLOGY
卷号	19 期号:1 页码:15
通讯作者	Gu, Wenhui([email protected]) ; Wang, Guangce([email protected])
摘要	Background Diatoms usually dominate phytoplankton blooms in open oceans, exhibiting extremely high population densities. Although the iron uptake rate of diatoms largely determines the magnitude and longevity of diatom blooms, the underlying mechanisms regulating iron uptake remain unclear. Results The transcription of two iron uptake proteins, ISIP2a and ISIP1, in the marine diatom Phaeodactylum tricornutum was enhanced with increasing cell density, whereas the cellular iron content showed the opposite trend. When compared with the wild-type strain, knockdown of ISIP2a resulted in 43% decrease in cellular iron content, implying the involvement of ISIP2a in iron uptake under high-cell density conditions. Incubation of the diatom cells with sonicated cell lysate conditioned by different cell densities did not affect ISIP2a and ISIP1 expression, ruling out regulation via chemical cues. In contrast, ISIP2a and ISIP1 transcription were strongly induced by red light. Besides, chlorophyll fluorescence excited from the blue light was also positively correlated with population density. Subsequently, a "sandwich" illumination incubator was designed to filter out stray light and ensure that the inner layer cells only receive the emitted chlorophyll fluorescence from outer layers, and the results showed that the increase in outer cell density significantly elevated ISIP2a and ISIP1 transcription in inner layer cells. In situ evidence from Tara oceans also showed positively correlated between diatom ISIP transcripts and chlorophyll content. Conclusions This study shows that chlorophyll fluorescence derived from neighboring cells is able to upregulate ISIP2a and ISIP1 expression to facilitate iron assimilation under high-cell density. These results provide novel insights into biotic signal sensing in phytoplankton, which can help to elucidate the underlying mechanisms of marine diatom blooms.
关键词	Chlorophyll fluorescence Density-dependent Iron uptake Biotic signal
DOI	10.1186/s12915-021-01177-z
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901500]; National Natural Science Foundation of China[41806171]; National Natural Science Foundation of China[41876163]; Key Deployment Project of the Centre for Ocean Mega-Research of Science; Chinese Academy of Sciences
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
WOS记录号	WOS:000721865800001
出版者	BMC
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177246
专题	实验海洋生物学重点实验室海洋生物技术研发中心
通讯作者	Gu, Wenhui; Wang, Guangce
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Publ Technol Serv Ctr, Inst Oceanol, Qingdao 266071, Peoples R China 6.Shenzhen Univ, Inst Adv Study, Shenzhen, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Liu, Xuehua,Xie, Xiujun,Gao, Shan,et al. Chlorophyll fluorescence as a light signal enhances iron uptake by the marine diatom Phaeodactylum tricornutum under high-cell density conditions[J]. BMC BIOLOGY,2021,19(1):15.
APA	Liu, Xuehua.,Xie, Xiujun.,Gao, Shan.,Wang, Lepu.,Zhou, Lu.,...&Wang, Guangce.(2021).Chlorophyll fluorescence as a light signal enhances iron uptake by the marine diatom Phaeodactylum tricornutum under high-cell density conditions.BMC BIOLOGY,19(1),15.
MLA	Liu, Xuehua,et al."Chlorophyll fluorescence as a light signal enhances iron uptake by the marine diatom Phaeodactylum tricornutum under high-cell density conditions".BMC BIOLOGY 19.1(2021):15.

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